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BMRB: 25866

2N8U

Solution Structure of the rNedd4 WW2 Domain by NMR

Authors

Spagnol, G., Kieken, F., Sorgen, P.L.

Assembly

rNedd4 WW2 Domain

Entity

1. rNedd4 WW2 Domain (polymer, Thiol state: not present), 39 monomers, 4430.648 Da Detail

GSSSGLPPGW EEKQDDRGRS YYVDHNSKTT TWSKPTMED

Formula weight

4430.648 Da

Source organism

Rattus norvegicus

Exptl. method

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.3 %, Completeness: 83.3 %, Completeness (bb): 75.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.3 % (364 of 437)	90.4 % (208 of 230)	71.9 % (120 of 167)	90.0 % (36 of 40)
Backbone	75.4 % (172 of 228)	89.9 % (71 of 79)	61.1 % (69 of 113)	88.9 % (32 of 36)
Sidechain	92.2 % (225 of 244)	90.7 % (137 of 151)	94.4 % (84 of 89)	100.0 % (4 of 4)
Aromatic	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (20 of 20)	100.0 % (2 of 2)
Methyl	100.0 % (16 of 16)	100.0 % (8 of 8)	100.0 % (8 of 8)

1. rNedd4 WW2

GSSSGLPPGW EEKQDDRGRS YYVDHNSKTT TWSKPTMED

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	rNedd4 WW2	[U-98% 13C; U-98% 15N]		1 mM
2	potassium phosphate	natural abundance		1.8 mM
3	DTT	natural abundance		1 mM
4	sodium chloride	natural abundance		137 mM
5	potassium chloride	natural abundance		2.7 mM
6	sodium phosphate	natural abundance		10 mM

LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 9 models in PDB: 2N8U, Strand ID: A Detail

Release date

2016-02-21

Citation

Structural Studies of the Nedd4 WW Domains and their Selectivity for the Cx43 Carboxyl-terminus
Spagnol, G., Kieken, F., Kopanic, J.L., Li, H., Zach, S., Stauch, K.L., Grosely, R., Sorgen, P.L.
J. Biol. Chem. (2016), 291, 7637-7650, PubMed 26841867 , DOI 10.1074/jbc.M115.701417 , Abstract

Related entities 1. rNedd4 WW2 Domain, : 1 : 1 : 344 entities Detail

Interaction partners 1. rNedd4 WW2 Domain, : 25 interactors Detail

More details for 25 interactors identified by 10 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	rNedd4 WW2	[U-98% 13C; U-98% 15N]		1 mM
2	potassium phosphate	natural abundance		1.8 mM
3	DTT	natural abundance		1 mM
4	sodium chloride	natural abundance		137 mM
5	potassium chloride	natural abundance		2.7 mM
6	sodium phosphate	natural abundance		10 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	rNedd4 WW2	[U-98% 13C; U-98% 15N]		1 mM
2	potassium phosphate	natural abundance		1.8 mM
3	DTT	natural abundance		1 mM
4	sodium chloride	natural abundance		137 mM
5	potassium chloride	natural abundance		2.7 mM
6	sodium phosphate	natural abundance		10 mM

3 3D HNHA

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	rNedd4 WW2	[U-98% 13C; U-98% 15N]		1 mM
2	potassium phosphate	natural abundance		1.8 mM
3	DTT	natural abundance		1 mM
4	sodium chloride	natural abundance		137 mM
5	potassium chloride	natural abundance		2.7 mM
6	sodium phosphate	natural abundance		10 mM

4 3D HNCACB

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	rNedd4 WW2	[U-98% 13C; U-98% 15N]		1 mM
2	potassium phosphate	natural abundance		1.8 mM
3	DTT	natural abundance		1 mM
4	sodium chloride	natural abundance		137 mM
5	potassium chloride	natural abundance		2.7 mM
6	sodium phosphate	natural abundance		10 mM

5 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	rNedd4 WW2	[U-98% 13C; U-98% 15N]		1 mM
2	potassium phosphate	natural abundance		1.8 mM
3	DTT	natural abundance		1 mM
4	sodium chloride	natural abundance		137 mM
5	potassium chloride	natural abundance		2.7 mM
6	sodium phosphate	natural abundance		10 mM

6 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	rNedd4 WW2	[U-98% 13C; U-98% 15N]		1 mM
2	potassium phosphate	natural abundance		1.8 mM
3	DTT	natural abundance		1 mM
4	sodium chloride	natural abundance		137 mM
5	potassium chloride	natural abundance		2.7 mM
6	sodium phosphate	natural abundance		10 mM

7 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	rNedd4 WW2	[U-98% 13C; U-98% 15N]		1 mM
2	potassium phosphate	natural abundance		1.8 mM
3	DTT	natural abundance		1 mM
4	sodium chloride	natural abundance		137 mM
5	potassium chloride	natural abundance		2.7 mM
6	sodium phosphate	natural abundance		10 mM

8 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	rNedd4 WW2	[U-98% 13C; U-98% 15N]		1 mM
2	potassium phosphate	natural abundance		1.8 mM
3	DTT	natural abundance		1 mM
4	sodium chloride	natural abundance		137 mM
5	potassium chloride	natural abundance		2.7 mM
6	sodium phosphate	natural abundance		10 mM

nullKeywords rNedd4 WW2