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BMRB: 25881

2N95

Chemical shift assignment of yeast Hit1 protein zinc finger

Authors

Bragantini, B., Quinternet, M., Manival, X.

Assembly

yeast Hit1 protein zinc finger

Entity

1. yeast Hit1 protein zinc finger, entity 1 (polymer, Thiol state: all other bound), 49 monomers, 5386.283 Da Detail

GPHMVSSAVK CGICRGVDGK YKCPKCGVRY CSLKCYKDAA KHVHKESEQ

2. ZINC ION (non-polymer), 65.409 × 2 Da

Total weight

5517.101 Da

Max. entity weight

5386.283 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 95.9 %, Completeness: 87.5 %, Completeness (bb): 91.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.5 % (484 of 553)	86.0 % (251 of 292)	89.2 % (190 of 213)	89.6 % (43 of 48)
Backbone	91.7 % (266 of 290)	90.1 % (91 of 101)	93.7 % (133 of 142)	89.4 % (42 of 47)
Sidechain	84.4 % (259 of 307)	83.8 % (160 of 191)	85.2 % (98 of 115)	100.0 % (1 of 1)
Aromatic	55.6 % (20 of 36)	55.6 % (10 of 18)	55.6 % (10 of 18)
Methyl	88.2 % (30 of 34)	88.2 % (15 of 17)	88.2 % (15 of 17)

1. entity 1

GPHMVSSAVK CGICRGVDGK YKCPKCGVRY CSLKCYKDAA KHVHKESEQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	2.2 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	DTT	[U-2H]	3 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Chem. Shift Complete2

Sequence coverage: 91.8 %, Completeness: 80.9 %, Completeness (bb): 81.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.9 % (895 of 1106)	83.4 % (487 of 584)	76.3 % (325 of 426)	86.5 % (83 of 96)
Backbone	81.9 % (475 of 580)	87.6 % (177 of 202)	76.4 % (217 of 284)	86.2 % (81 of 94)
Sidechain	81.4 % (500 of 614)	81.2 % (310 of 382)	81.7 % (188 of 230)	100.0 % (2 of 2)
Aromatic	55.6 % (40 of 72)	55.6 % (20 of 36)	55.6 % (20 of 36)
Methyl	88.2 % (60 of 68)	88.2 % (30 of 34)	88.2 % (30 of 34)

1. entity 1

GPHMVSSAVK CGICRGVDGK YKCPKCGVRY CSLKCYKDAA KHVHKESEQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	2.2 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	DTT	[U-2H]	3 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N95, Strand ID: A Detail

Release date

2016-05-22

Citation

Functional and Structural Insights of the Zinc-Finger HIT protein family members Involved in Box C/D snoRNP Biogenesis
Bragantini, B., Quinternet, M., Manival, X., Charpentier, B., Tiotiu, D., Rothe, B., Saliou, J., Cianferani, S.
J. Mol. Biol. (2016), 428, 2488-2506, PubMed 27139642 , DOI 10.1016/j.jmb.2016.04.028 , Abstract

Related entities 1. yeast Hit1 protein zinc finger, entity 1, : 1 : 2 : 22 entities Detail

Interaction partners 1. yeast Hit1 protein zinc finger, entity 1, : 4 interactors Detail

More details for 4 interactors identified by 1 citations

Experiments performed 19 experiments Detail

1 2D 1H-1H NOESY