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BMRB: 25883

2N97

DD homodimer

Authors

Lin, Z., Ibanez, C.F.

Assembly

DD homodimer

Entity

1. DD homodimer (polymer, Thiol state: all free), 94 monomers, 10161.24 × 2 Da Detail

GLYSSLPPAK REEVEKLLNG SAGDTWRHLA GELGYQPEHI DSFTHEACPV RALLASWATQ DSATLDALLA ALRRIQRADL VESLCSESTA TSPV

Total weight

20322.48 Da

Max. entity weight

10161.24 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 84.0 %, Completeness (bb): 81.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.0 % (865 of 1030)	90.0 % (475 of 528)	72.5 % (295 of 407)	100.0 % (95 of 95)
Backbone	81.9 % (454 of 554)	93.6 % (176 of 188)	68.2 % (189 of 277)	100.0 % (89 of 89)
Sidechain	88.3 % (499 of 565)	87.9 % (299 of 340)	88.6 % (194 of 219)	100.0 % (6 of 6)
Aromatic	32.3 % (20 of 62)	32.3 % (10 of 31)	27.6 % (8 of 29)	100.0 % (2 of 2)
Methyl	99.2 % (117 of 118)	98.3 % (58 of 59)	100.0 % (59 of 59)

1. entity

GLYSSLPPAK REEVEKLLNG SAGDTWRHLA GELGYQPEHI DSFTHEACPV RALLASWATQ DSATLDALLA ALRRIQRADL VESLCSESTA TSPV

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	p75NTR DD	[U-99% 13C; U-99% 15N]	1 mM
2	DTT	[U-98% 2H]	10 mM
3	p75NTR DD	natural abundance	1 mM
4	sodium phosphate	natural abundance	50 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2N97, Strand ID: A, B Detail

Release date

2015-12-20

Citation

Structural basis of death domain signaling in the p75 neurotrophin receptor
Lin, Z., Tann, J.Y., Goh, E.TH., Kelly, C., Lim, K.B., Gao, J.F., Ibanez, C.F.
Elife (2015), 4, 11692-11692, PubMed 26646181 , DOI 10.7554/eLife.11692 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 25828 released on 2015-12-20
    Title RIP2 CARD
  BMRB: 25829 released on 2015-12-20
    Title p75NTR DD:RhoGDI
  BMRB: 25833 released on 2015-12-20
    Title p75NTR DD:RIP2 CARD

Related entities 1. DD homodimer, : 1 : 4 : 28 entities Detail

Interaction partners 1. DD homodimer, : 18 interactors Detail

More details for 18 interactors identified by 7 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC