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BMRB: 25902

6C0A

actinin-1 EF hand 3,4 Bound to Cav1.2 IQ Motif

Authors

Turner, M.L., Ames, J.B.

Assembly

actinin-1 EF bound to IQ

Entity

1. actinin-1 (polymer, Thiol state: all free), 89 monomers, 9784.818 Da Detail

MGSSHHHHHS SGLVPRGSHM DTADQVMASF KILAGDKNYI TMDELRRELP PDQAEYCIAR MAPYTGPDSV PGALDYMSFS TALYGESDL

2. IQ-Motif (polymer, Thiol state: not present), 25 monomers, 3113.608 Da Detail

TVGKFYATFL IQEYFRKFKK RKEQG

Total weight

12898.426 Da

Max. entity weight

9784.818 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 61.4 %, Completeness: 51.4 %, Completeness (bb): 59.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	51.4 % (678 of 1319)	51.2 % (351 of 686)	50.4 % (262 of 520)	57.5 % (65 of 113)
Backbone	59.2 % (398 of 672)	57.6 % (133 of 231)	60.1 % (200 of 333)	60.2 % (65 of 108)
Sidechain	45.7 % (344 of 752)	47.9 % (218 of 455)	43.2 % (126 of 292)	0.0 % (0 of 5)
Aromatic	10.0 % (14 of 140)	18.6 % (13 of 70)	1.4 % (1 of 70)
Methyl	67.0 % (63 of 94)	70.2 % (33 of 47)	63.8 % (30 of 47)

1. actinin-1

MGSSHHHHHS SGLVPRGSHM DTADQVMASF KILAGDKNYI TMDELRRELP PDQAEYCIAR MAPYTGPDSV PGALDYMSFS TALYGESDL

2. IQ-Motif

TVGKFYATFL IQEYFRKFKK RKEQG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details actinin-1 EF hand c-lobe bound to Cav1.2 IQ-Motif

#	Name	Isotope labeling	Concentration
1	actinin-1 EF hand 3,4	[U-99% 15N]	0.5 mM
2	Cav1.2 IQ-Motif	natural abundance	0.5 mM
3	Tris	natural abundance	20 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details actinin-1 EF hand 3,4 bound to Cav1.2 IQ-Motif

#	Name	Isotope labeling	Concentration
4	actinin-1 EF hand 3,4	[U-99% 13C; U-99% 15N]	0.5 mM
5	Cav1.2 IQ-Motif	natural abundance	0.5 mM
6	Tris	natural abundance	20 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details actinin-1 EF hand 3,4 bound to Cav1.2 IQ-Motif

#	Name	Isotope labeling	Concentration
7	actinin-1 EF hand 3,4	[U-99% 13C; U-99% 15N]	0.5 mM
8	Cav1.2 IQ-Motif	natural abundance	0.5 mM
9	Tris	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 4 models in PDB: 6C0A, Strand ID: A, B Detail

Release date

2016-10-26

Citation

Chemical shift assignments of the C-terminal EF-hand domain of α-actinin-1
Turner, M.L., Anderson, D.E., Rajan, S., Hell, J.W., Ames, J.B.
Biomol. NMR Assign. (2016), 10, 219-222, PubMed 26861220 , DOI 10.1007/s12104-016-9670-2 , Abstract

Related entities 1. actinin-1, : 13 entities Detail