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BMRB: 25906


25906
2N9H
Glucose as a nuclease mimic in DNA
Authors
Gomez-Pinto, I., Vengut-Climent, E., Lucas, R., Avino, A., Eritja, R., Gonzalez-Ibanez, C., Morales, J., Muro, A., Penalver, P., Fonseca-Guerra, C., Bickelhaupt, M.
Assembly
Glucose as a nuclease mimic in DNA
Entity
1. DNA (5'-D(*GP*AP*TP*GP*AP*CP*TP*GP*CP*TP*AP*G)-3') (polymer, Thiol state: not present), 12 monomers, 3765.400 Da Detail

GATGACTGCT AG


2. DNA (5'-D(*CP*TP*AP*GP*CP*(GL6)P*GP*TP*CP*AP*TP*C)-3') (polymer, Thiol state: not present), 12 monomers, 3672.345 Da Detail

CTAGCXGTCA TC


Total weight
7437.745 Da
Max. entity weight
3765.4 Da
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.8 %, Completeness: 89.8 %, Completeness (bb): 91.3 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All89.8 % (202 of 225)89.8 % (202 of 225)
Suger, PO491.3 % (147 of 161)91.3 % (147 of 161)
Nucleobase85.9 % (55 of 64)85.9 % (55 of 64)
Aromatic93.5 % (43 of 46)93.5 % (43 of 46)
Methyl100.0 % (6 of 6)100.0 % (6 of 6)

1. DNA (5'-D(*GP*AP*TP*GP*AP*CP*TP*GP*CP*TP*AP*G)-3')

GATGACTGCT AG

2. DNA (5'-D(*CP*TP*AP*GP*CP*(GL6)P*GP*TP*CP*AP*TP*C)-3')

CTAGCXGTCA TC

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 279.6 K, pH 7


#NameIsotope labelingTypeConcentration
1DNA (5'-D(*GP*AP*TP*GP*AP*CP*TP*GP*CP*TP*AP*G)-3')natural abundance1 mM
2DNA (5'-D(*CP*TP*AP*GP*CP*(GL6)P*GP*TP*CP*AP*TP*C)-3')natural abundance1 mM
3SUGARnatural abundance1 mM
4Potassium phosphatenatural abundance10 mM

Release date
2016-08-24
Citation
Glucose-Nucleobase Pseudo Base Pairs: Biomolecular Interactions within DNA
Vengut-Climent, E., Gomez-Pinto, I., Lucas, R., Penalver, P., Avino, A., Fonseca-Guerra, C., Bickelhaupt, M., Eritja, R., Gonzalez-Ibanez, C., Morales, J.
Angew. Chem Int Ed Engl. (2016), 55, 8643-8647, PubMed 27328804 , DOI 10.1002/anie.201603510 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 25903 released on 2016-08-24
    Title Glucose as non natural nucleobase
Experiments performed 4 experiments Detail
NMR combined restraints 3 contents Detail
Keywords Carbohydrate-DNA interaction, Glucose-nucleobase DNA structure