1H, 13C, and 15N Chemical Shift Assignments for in-cell GB1
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.7 % (548 of 632) | 85.0 % (272 of 320) | 87.6 % (219 of 250) | 91.9 % (57 of 62) |
Backbone | 97.1 % (332 of 342) | 97.5 % (116 of 119) | 96.4 % (160 of 166) | 98.2 % (56 of 57) |
Sidechain | 77.2 % (264 of 342) | 77.6 % (156 of 201) | 78.7 % (107 of 136) | 20.0 % (1 of 5) |
Aromatic | 60.7 % (34 of 56) | 60.7 % (17 of 28) | 59.3 % (16 of 27) | 100.0 % (1 of 1) |
Methyl | 83.3 % (55 of 66) | 84.8 % (28 of 33) | 81.8 % (27 of 33) |
1. entity
MGTYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Protein G B1 | [U-100% 13C; U-100% 15N] | 250 uM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % |