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BMRB: 25914

2N9P

Solution structure of RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain

Authors

Martinez-Lumbreras, S., Krysztofinska, E.M., Thapaliya, A., Isaacson, R.L.

Assembly

RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain

Entity

1. RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain, entity 1 (polymer, Thiol state: free and other bound), 42 monomers, 4700.356 Da Detail

GSMAEASPHP GRYFCHCCSV EIVPRLPDYI CPRCESGFIE EL

2. RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain, entity 2 (polymer, Thiol state: not present), 100 monomers, 11255.62 Da Detail

MAHHHHHHVD DDDKMLEVLV KTLDSQTRTF IVGAQMNVKE FKEHIAASVS IPSEKQRLIY QGRVLQDDKK LQEYNVGGKV IHLVERAPPQ THLPSGASSG

3. ZINC ION (non-polymer), 65.409 Da

Total weight

16021.386 Da

Max. entity weight

11255.62 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 43.0 %, Completeness: 36.8 %, Completeness (bb): 39.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	36.8 % (600 of 1632)	36.2 % (308 of 851)	37.2 % (238 of 639)	38.0 % (54 of 142)
Backbone	39.2 % (327 of 834)	39.4 % (112 of 284)	39.1 % (163 of 417)	39.1 % (52 of 133)
Sidechain	35.1 % (327 of 931)	34.6 % (196 of 567)	36.3 % (129 of 355)	22.2 % (2 of 9)
Aromatic	37.1 % (43 of 116)	37.9 % (22 of 58)	36.2 % (21 of 58)
Methyl	36.1 % (52 of 144)	36.1 % (26 of 72)	36.1 % (26 of 72)

1. entity 1

GSMAEASPHP GRYFCHCCSV EIVPRLPDYI CPRCESGFIE EL

2. entity 2

MAHHHHHHVD DDDKMLEVLV KTLDSQTRTF IVGAQMNVKE FKEHIAASVS IPSEKQRLIY QGRVLQDDKK LQEYNVGGKV IHLVERAPPQ THLPSGASSG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	600 uM
2	entity_2	natural abundance	800 uM
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	TCEP	natural abundance	250 uM
6	DSS	natural abundance	10 uM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	entity_1	[U-99% 13C; U-99% 15N]	600 uM
10	entity_2	natural abundance	800 uM
11	potassium phosphate	natural abundance	10 mM
12	sodium chloride	natural abundance	100 mM
13	TCEP	natural abundance	250 uM
14	DSS	natural abundance	10 uM
15	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	entity_1	natural abundance	600 uM
17	entity_2	[U-99% 13C; U-99% 15N]	400 uM
18	potassium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	100 mM
20	TCEP	natural abundance	250 uM
21	DSS	natural abundance	10 uM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
24	entity_1	natural abundance	600 uM
25	entity_2	[U-99% 13C; U-99% 15N]	400 uM
26	potassium phosphate	natural abundance	10 mM
27	sodium chloride	natural abundance	100 mM
28	TCEP	natural abundance	250 uM
29	DSS	natural abundance	10 uM
30	D2O	natural abundance	100 %

Chem. Shift Complete2

Sequence coverage: 66.9 %, Completeness: 48.1 %, Completeness (bb): 51.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.1 % (1571 of 3264)	47.6 % (811 of 1702)	47.6 % (608 of 1278)	53.5 % (152 of 284)
Backbone	51.0 % (850 of 1668)	52.1 % (296 of 568)	49.5 % (413 of 834)	53.0 % (141 of 266)
Sidechain	46.0 % (856 of 1862)	45.4 % (515 of 1134)	46.5 % (330 of 710)	61.1 % (11 of 18)
Aromatic	33.2 % (77 of 232)	34.5 % (40 of 116)	31.9 % (37 of 116)
Methyl	56.9 % (164 of 288)	57.6 % (83 of 144)	56.3 % (81 of 144)

1. entity 1

GSMAEASPHP GRYFCHCCSV EIVPRLPDYI CPRCESGFIE EL

2. entity 2

MAHHHHHHVD DDDKMLEVLV KTLDSQTRTF IVGAQMNVKE FKEHIAASVS IPSEKQRLIY QGRVLQDDKK LQEYNVGGKV IHLVERAPPQ THLPSGASSG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	600 uM
2	entity_2	natural abundance	800 uM
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	TCEP	natural abundance	250 uM
6	DSS	natural abundance	10 uM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	entity_1	[U-99% 13C; U-99% 15N]	600 uM
10	entity_2	natural abundance	800 uM
11	potassium phosphate	natural abundance	10 mM
12	sodium chloride	natural abundance	100 mM
13	TCEP	natural abundance	250 uM
14	DSS	natural abundance	10 uM
15	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	entity_1	natural abundance	600 uM
17	entity_2	[U-99% 13C; U-99% 15N]	400 uM
18	potassium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	100 mM
20	TCEP	natural abundance	250 uM
21	DSS	natural abundance	10 uM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
24	entity_1	natural abundance	600 uM
25	entity_2	[U-99% 13C; U-99% 15N]	400 uM
26	potassium phosphate	natural abundance	10 mM
27	sodium chloride	natural abundance	100 mM
28	TCEP	natural abundance	250 uM
29	DSS	natural abundance	10 uM
30	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N9P, Strand ID: A, C Detail

Release date

2016-05-22

Citation

Structural and functional insights into the E3 ligase, RNF126
Krysztofinska, E.M., Martinez-Lumbreras, S., Thapaliya, A., Evans, N.J., High, S., Isaacson, R.L.
Sci. Rep. (2016), 6, 26433-26433, PubMed 27193484 , DOI 10.1038/srep26433 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25913 released on 2016-05-22
Title Solution structure of RNF126 N-terminal zinc finger domain

Related entities 1. RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain, entity 1, : 6 : 34 entities Detail

Related entities 2. RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain, entity 2, : 17 entities Detail

Interaction partners 1. RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain, entity 1, : 29 interactors Detail

More details for 29 interactors identified by 14 citations

Experiments performed 20 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	600 uM
2	entity_2	natural abundance	800 uM
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	TCEP	natural abundance	250 uM
6	DSS	natural abundance	10 uM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	entity_1	[U-99% 13C; U-99% 15N]	600 uM
10	entity_2	natural abundance	800 uM
11	potassium phosphate	natural abundance	10 mM
12	sodium chloride	natural abundance	100 mM
13	TCEP	natural abundance	250 uM
14	DSS	natural abundance	10 uM
15	D2O	natural abundance	100 %

3 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	600 uM
2	entity_2	natural abundance	800 uM
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	TCEP	natural abundance	250 uM
6	DSS	natural abundance	10 uM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	600 uM
2	entity_2	natural abundance	800 uM
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	TCEP	natural abundance	250 uM
6	DSS	natural abundance	10 uM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

5 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	600 uM
2	entity_2	natural abundance	800 uM
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	TCEP	natural abundance	250 uM
6	DSS	natural abundance	10 uM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

6 3D HN(CA)CO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	600 uM
2	entity_2	natural abundance	800 uM
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	TCEP	natural abundance	250 uM
6	DSS	natural abundance	10 uM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	600 uM
2	entity_2	natural abundance	800 uM
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	TCEP	natural abundance	250 uM
6	DSS	natural abundance	10 uM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	entity_1	[U-99% 13C; U-99% 15N]	600 uM
10	entity_2	natural abundance	800 uM
11	potassium phosphate	natural abundance	10 mM
12	sodium chloride	natural abundance	100 mM
13	TCEP	natural abundance	250 uM
14	DSS	natural abundance	10 uM
15	D2O	natural abundance	100 %

9 3D 12C14N filtered 13C edited NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	entity_1	[U-99% 13C; U-99% 15N]	600 uM
10	entity_2	natural abundance	800 uM
11	potassium phosphate	natural abundance	10 mM
12	sodium chloride	natural abundance	100 mM
13	TCEP	natural abundance	250 uM
14	DSS	natural abundance	10 uM
15	D2O	natural abundance	100 %

10 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	entity_1	[U-99% 13C; U-99% 15N]	600 uM
10	entity_2	natural abundance	800 uM
11	potassium phosphate	natural abundance	10 mM
12	sodium chloride	natural abundance	100 mM
13	TCEP	natural abundance	250 uM
14	DSS	natural abundance	10 uM
15	D2O	natural abundance	100 %

11 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	entity_1	natural abundance	600 uM
17	entity_2	[U-99% 13C; U-99% 15N]	400 uM
18	potassium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	100 mM
20	TCEP	natural abundance	250 uM
21	DSS	natural abundance	10 uM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

12 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
24	entity_1	natural abundance	600 uM
25	entity_2	[U-99% 13C; U-99% 15N]	400 uM
26	potassium phosphate	natural abundance	10 mM
27	sodium chloride	natural abundance	100 mM
28	TCEP	natural abundance	250 uM
29	DSS	natural abundance	10 uM
30	D2O	natural abundance	100 %

13 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	entity_1	natural abundance	600 uM
17	entity_2	[U-99% 13C; U-99% 15N]	400 uM
18	potassium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	100 mM
20	TCEP	natural abundance	250 uM
21	DSS	natural abundance	10 uM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

14 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	entity_1	natural abundance	600 uM
17	entity_2	[U-99% 13C; U-99% 15N]	400 uM
18	potassium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	100 mM
20	TCEP	natural abundance	250 uM
21	DSS	natural abundance	10 uM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

15 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	entity_1	natural abundance	600 uM
17	entity_2	[U-99% 13C; U-99% 15N]	400 uM
18	potassium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	100 mM
20	TCEP	natural abundance	250 uM
21	DSS	natural abundance	10 uM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

16 3D HN(CA)CO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	entity_1	natural abundance	600 uM
17	entity_2	[U-99% 13C; U-99% 15N]	400 uM
18	potassium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	100 mM
20	TCEP	natural abundance	250 uM
21	DSS	natural abundance	10 uM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

17 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	entity_1	natural abundance	600 uM
17	entity_2	[U-99% 13C; U-99% 15N]	400 uM
18	potassium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	100 mM
20	TCEP	natural abundance	250 uM
21	DSS	natural abundance	10 uM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

18 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
24	entity_1	natural abundance	600 uM
25	entity_2	[U-99% 13C; U-99% 15N]	400 uM
26	potassium phosphate	natural abundance	10 mM
27	sodium chloride	natural abundance	100 mM
28	TCEP	natural abundance	250 uM
29	DSS	natural abundance	10 uM
30	D2O	natural abundance	100 %

19 3D 12C14N filtered 13C edited NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
24	entity_1	natural abundance	600 uM
25	entity_2	[U-99% 13C; U-99% 15N]	400 uM
26	potassium phosphate	natural abundance	10 mM
27	sodium chloride	natural abundance	100 mM
28	TCEP	natural abundance	250 uM
29	DSS	natural abundance	10 uM
30	D2O	natural abundance	100 %

20 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
24	entity_1	natural abundance	600 uM
25	entity_2	[U-99% 13C; U-99% 15N]	400 uM
26	potassium phosphate	natural abundance	10 mM
27	sodium chloride	natural abundance	100 mM
28	TCEP	natural abundance	250 uM
29	DSS	natural abundance	10 uM
30	D2O	natural abundance	100 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_25914_2n9p.nef
Input source #2: Coordindates	2n9p.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:34:CYS:SG	3:1:ZN:ZN	unknown	unknown	n/a
1:15:CYS:SG	3:1:ZN:ZN	unknown	unknown	n/a
1:18:CYS:SG	3:1:ZN:ZN	unknown	unknown	n/a
1:31:CYS:SG	3:1:ZN:ZN	unknown	unknown	n/a

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
B	1	ZN	ZINC ION	None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-0--------10--------20--------30--------40
GSMAEASPHPGRYFCHCCSVEIVPRLPDYICPRCESGFIEEL
||||||||||||||||||||||||||||||||||||||||||
GSMAEASPHPGRYFCHCCSVEIVPRLPDYICPRCESGFIEEL
--------10--------20--------30--------40--

Entity_assembly_ID (NMR): C vs Auth_asym_ID (model): C

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	42	0	0	100.0
C	C	100	0	0	100.0

Content subtype: combined_25914_2n9p.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	4		No information
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	452		100.0 (chain: A, length: 42)
2	Assigned chemical shifts	assigned_chem_shift_list_2	Warning	950		93.0 (chain: C, length: 100)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	7895 (1)	noe	90.5 (chain: A, length: 42), 91.0 (chain: C, length: 100)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 42, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 233
  - ¹³C chemical shifts: 181
  - ¹⁵N chemical shifts: 38
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
Saveframe: assigned_chem_shift_list_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: C, Chain length: 100, Sequence coverage: 93.0%
- Total number of assignments
  - ¹H chemical shifts: 485
  - ¹³C chemical shifts: 367
  - ¹⁵N chemical shifts: 98
- Completeness of assignments
  - Entity_assemble_ID: C
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: C

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	245	233	95.1
¹³C chemical shifts	187	181	96.8
¹⁵N chemical shifts	40	38	95.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	82	79	96.3
¹³C chemical shifts	84	82	97.6
¹⁵N chemical shifts	37	35	94.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	163	154	94.5
¹³C chemical shifts	103	99	96.1
¹⁵N chemical shifts	3	3	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	17	17	100.0
¹³C chemical shifts	17	17	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	20	90.9
¹³C chemical shifts	22	19	86.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	606	485	80.0
¹³C chemical shifts	452	367	81.2
¹⁵N chemical shifts	109	98	89.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	202	181	89.6
¹³C chemical shifts	200	166	83.0
¹⁵N chemical shifts	96	87	90.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	404	304	75.2
¹³C chemical shifts	252	201	79.8
¹⁵N chemical shifts	13	11	84.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	55	93.2
¹³C chemical shifts	59	55	93.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	36	16	44.4
¹³C chemical shifts	36	16	44.4

Covalent bonds

Saveframe: assembly
- Status: OK

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 42, Sequence coverage: 90.5%
  - Entity_assembly_ID: C, Chain length: 100, Sequence coverage: 91.0%
- Total number of restraints: 7895
- Weight of restraints: 1.0 (7895)
- Potential type of restraints: square-well-parabolic (7895)
- Range of distance target values: 1.56, 5.52 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
    None
  - Chain_ID: C
- Distance restraints on contact map

Keywords BAG6, E3 ligase, RNF126, UBL, zinc finger

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Found 1 Document (1.446 seconds)

BMRB: 25914

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Entries sharing articles BMRB: 1 entries Detail

Related entities 1. RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain, entity 1, : 6 : 34 entities Detail

Related entities 2. RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain, entity 2, : 17 entities Detail

Interaction partners 1. RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain, entity 1, : 29 interactors Detail

Experiments performed 20 experiments Detail

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