BMRBj - BMREntryMaster

Found 1 Document (1.145 seconds)

BMRB: 25918

2N9U

Solution NMR structure of Erythrobacter litoralis PhyR response regulator REC domain

Authors

Correa, F., Gardner, K.H.

Assembly

Erythrobacter litoralis PhyR response regulator REC domain

Entity

1. Erythrobacter litoralis PhyR response regulator REC domain (polymer, Thiol state: not present), 129 monomers, 13814.45 Da Detail

GAMGSTNVLI IEDEPLISMQ LEDLVRSLGH DIAGTAATRT QAQEAVAKEK PGLVLADIQL ADGSSGIDAV EDILGQFDVP VIFITAYPER LLTGDRPEPT YLVTKPFQES TVRTTISQAL FFQNSPTAV

Formula weight

13814.45 Da

Source organism

Tuberaphis taiwana

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.8 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.8 % (1404 of 1436)	97.3 % (715 of 735)	98.2 % (560 of 570)	98.5 % (129 of 131)
Backbone	97.8 % (741 of 758)	96.9 % (251 of 259)	97.9 % (370 of 378)	99.2 % (120 of 121)
Sidechain	98.1 % (783 of 798)	97.5 % (464 of 476)	99.4 % (310 of 312)	90.0 % (9 of 10)
Aromatic	97.1 % (68 of 70)	94.3 % (33 of 35)	100.0 % (35 of 35)
Methyl	100.0 % (182 of 182)	100.0 % (91 of 91)	100.0 % (91 of 91)

1. entity

GAMGSTNVLI IEDEPLISMQ LEDLVRSLGH DIAGTAATRT QAQEAVAKEK PGLVLADIQL ADGSSGIDAV EDILGQFDVP VIFITAYPER LLTGDRPEPT YLVTKPFQES TVRTTISQAL FFQNSPTAV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 K, pH 7

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
2	TRIS	natural abundance	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	90 mM
5	D2O	natural abundance	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.33 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N9U, Strand ID: A Detail

Release date

2016-08-31

Citation

Basis of Mutual Domain Inhibition in a Bacterial Response Regulator
Correa, F., Gardner, K.H.
Cell Chem. Biol. (2016), S2451-9456, 30240-30249, PubMed 27524295 , DOI 10.1016/j.chembiol.2016.07.010 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 26722 released on 2016-08-31
Title 1H, 15N, 13C backbone shift assignments of PhyR protein from Erythrobacter litoralis

Related entities 1. Erythrobacter litoralis PhyR response regulator REC domain, : 1 : 112 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC