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BMRB: 25919

2N9X

LC3 FUNDC1 complex structure

Authors

Xia, B., Kuang, Y.

Assembly

LC3 FUNDC1 complex

Entity

1. LC3 FUNDC1 complex, entity 1 (polymer, Thiol state: not present), 120 monomers, 14129.11 Da Detail

MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG

2. LC3 FUNDC1 complex, entity 2 (polymer, Thiol state: not present), 17 monomers, 2071.022 Da Detail

DYESDDDSYE VLDLTEY

Total weight

16200.133 Da

Max. entity weight

14129.11 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 89.2 %, Completeness (bb): 85.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.2 % (1493 of 1673)	95.2 % (835 of 877)	81.5 % (533 of 654)	88.0 % (125 of 142)
Backbone	85.7 % (694 of 810)	97.1 % (264 of 272)	77.6 % (316 of 407)	87.0 % (114 of 131)
Sidechain	92.0 % (916 of 996)	94.4 % (571 of 605)	87.9 % (334 of 380)	100.0 % (11 of 11)
Aromatic	82.6 % (114 of 138)	91.3 % (63 of 69)	73.9 % (51 of 69)
Methyl	86.5 % (128 of 148)	82.4 % (61 of 74)	90.5 % (67 of 74)

1. entity 1

MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG

2. entity 2

DYESDDDSYE VLDLTEY

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2N9X, Strand ID: A, B Detail

Release date

2015-12-16

Citation

Structural basis for the phosphorylation of FUNDC1 LIR as a molecular switch of mitophagy
Kuang, Y., Ma, K., Zhou, C., Ding, P., Zhu, Y., Chen, Q., Xia, B.
Autophagy (2016), 12, 2363-2373, PubMed 27653272 , DOI 10.1080/15548627.2016.1238552 , Abstract

Related entities 1. LC3 FUNDC1 complex, entity 1, : 1 : 2 : 1 : 78 entities Detail

Related entities 2. LC3 FUNDC1 complex, entity 2, : 1 : 2 : 1 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

4 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

5 3D HN(CO)CA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

6 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

7 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

8 3D HBHA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

9 3D HCCH-COSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

10 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

11 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

12 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

13 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

14 2D 1H-1H TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

15 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

16 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.9 mM
2	entity_2	natural abundance		1.8 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		25 mM
5	D2O	[U-2H]		10 %
6	DSS	natural abundance		1 %

nullKeywords LC3 interacting region (LIR), protein/peptide