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BMRB: 25920

2N9Y

Structure of the Integrin alphaIIb-beta3(A711P) Transmembrane Complex

Authors

Schmidt, T., Situ, A.J., Ulmer, T.S.

Assembly

Integrin alphaIIb-beta3(A711P) Transmembrane Complex

Entity

1. Integrin alphaIIb-beta3(A711P) Transmembrane Complex, entity 1 (polymer, Thiol state: all disulfide bound), 42 monomers, 4778.855 Da Detail

GALEERCIPI WWVLVGVLGG LLLLTILVLA MWKVGFFKRN RP

2. Integrin alphaIIb-beta3(A711P) Transmembrane Complex, entity 2 (polymer, Thiol state: all disulfide bound), 43 monomers, 4739.810 Da Detail

GESPKCPDIL VVLLSVMGAI LLIGLAPLLI WKLLITIHDR KEF

Total weight

9518.665 Da

Max. entity weight

4778.855 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	2:CYS6:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 41.8 %, Completeness (bb): 77.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	41.8 % (441 of 1054)	24.4 % (132 of 542)	54.1 % (231 of 427)	91.8 % (78 of 85)
Backbone	77.2 % (386 of 500)	51.4 % (89 of 173)	90.3 % (223 of 247)	92.5 % (74 of 80)
Sidechain	20.0 % (126 of 631)	11.7 % (43 of 369)	30.7 % (79 of 257)	80.0 % (4 of 5)
Aromatic	26.8 % (22 of 82)	34.1 % (14 of 41)	10.8 % (4 of 37)	100.0 % (4 of 4)
Methyl	9.6 % (15 of 156)	11.5 % (9 of 78)	7.7 % (6 of 78)

1. entity 1

GALEERCIPI WWVLVGVLGG LLLLTILVLA MWKVGFFKRN RP

2. entity 2

GESPKCPDIL VVLLSVMGAI LLIGLAPLLI WKLLITIHDR KEF

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	entity_1	[U-15N; U-2H]	0.8 mM
2	entity_2	natural abundance	0.8 mM
3	DHPC	natural abundance	350 mM
4	DMPC	natural abundance	105 mM
5	NaH2PO4/Na2HPO4	natural abundance	25 mM
6	NaN3	natural abundance	0.02 % w/v

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 7.4

#	Name	Isotope labeling	Concentration
7	entity_1	natural abundance	0.8 mM
8	entity_2	[U-15N; U-2H]	0.8 mM
9	DHPC	natural abundance	350 mM
10	DMPC	natural abundance	105 mM
11	NaH2PO4/Na2HPO4	natural abundance	25 mM
12	NaN3	natural abundance	0.02 % w/v

Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 7.4

#	Name	Isotope labeling	Concentration
13	entity_1	[U-99% 13C; U-99% 15N; 80% 2H]	0.8 mM
14	entity_2	[U-99% 13C; U-99% 15N; 80% 2H]	0.8 mM
15	DHPC	natural abundance	350 mM
16	DMPC	natural abundance	105 mM
17	NaH2PO4/Na2HPO4	natural abundance	25 mM
18	NaN3	natural abundance	0.02 % w/v

Protein Blocks Logo

Calculated from 21 models in PDB: 2N9Y, Strand ID: A, B Detail

Release date

2016-07-27

Citation

Structural and thermodynamic basis of proline-induced transmembrane complex stabilization
Schmidt, T., Situ, A.J., Ulmer, T.S.
Sci. Rep. (2016), 6, 29809-29809, PubMed 27436065 , DOI 10.1038/srep29809 , Abstract

Related entities 1. Integrin alphaIIb-beta3(A711P) Transmembrane Complex, entity 1, : 1 : 6 : 46 entities Detail

Related entities 2. Integrin alphaIIb-beta3(A711P) Transmembrane Complex, entity 2, : 1 : 8 : 33 entities Detail

Interaction partners 1. Integrin alphaIIb-beta3(A711P) Transmembrane Complex, entity 1, : 3 : 1 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 6 experiments Detail

1 3D 1H-15N NOESY