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BMRB: 25924

2NA2

Drosha Quad

Authors

Showalter, S.A., Sahu, D., Kranick, J.C.

Assembly

Drosha Quad

Entity

1. Drosha Quad (polymer), 82 monomers, 8961.241 Da Detail

GPGNDPISQL QQCCLTLRTE GKEPDIPLYK TLQTVGPSHA RTYTVAVYFK GERIGCGKGP SKKQAKMGAA MDALEKYNFP QM

Formula weight

8961.241 Da

Source organism

Exptl. method

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.5 %, Completeness: 70.7 %, Completeness (bb): 87.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.7 % (670 of 947)	60.6 % (303 of 500)	82.4 % (300 of 364)	80.7 % (67 of 83)
Backbone	87.2 % (417 of 478)	80.7 % (134 of 166)	91.1 % (216 of 237)	89.3 % (67 of 75)
Sidechain	59.0 % (320 of 542)	50.6 % (169 of 334)	75.5 % (151 of 200)	0.0 % (0 of 8)
Aromatic	10.7 % (6 of 56)	14.3 % (4 of 28)	7.1 % (2 of 28)
Methyl	83.3 % (60 of 72)	75.0 % (27 of 36)	91.7 % (33 of 36)

1. entity

GPGNDPISQL QQCCLTLRTE GKEPDIPLYK TLQTVGPSHA RTYTVAVYFK GERIGCGKGP SKKQAKMGAA MDALEKYNFP QM

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

LACS Plot; CA

Referencing offset: 2.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 2.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 2.83 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2NA2, Strand ID: A Detail

Release date

2016-01-20

Citation

Engineering double-stranded RNA binding activity into the Drosha double-stranded RNA binding domain results in a loss of microRNA processing function
Kranick, J.C., Chadalavada, D.M., Sahu, D., Showalter, S.A.
PLoS One (2017), 12, e0182445-e0182445, PubMed 28792523 , DOI 10.1371/journal.pone.0182445 , Abstract

Related entities 1. Drosha Quad, : 1 : 105 entities Detail

Interaction partners 1. Drosha Quad, : 29 interactors Detail

More details for 29 interactors identified by 15 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

2 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

3 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

6 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

7 3D (H)CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

8 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

10 3D HNHA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.29

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		850 mM
2	cacodylate pH 7.29	natural abundance		100 mM
3	potassium chloride	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_25924_2na2.nef
Input source #2: Coordindates	2na2.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--
GPGNDPISQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSKKQAKMGAAMDALEKYNFPQM
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GPGNDPISQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSKKQAKMGAAMDALEKYNFPQM

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	82	0	0	100.0

Content subtype: combined_25924_2na2.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	663		91.5 (chain: A, length: 82)
1	Distance restraints	CNS/XPLOR_distance_constraints_4	Warning	567 (1)	noe	90.2 (chain: A, length: 82)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	128 (128)	.	87.8 (chain: A, length: 82)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 82, Sequence coverage: 91.5%
- Total number of assignments
  - ¹H chemical shifts: 298
  - ¹³C chemical shifts: 298
  - ¹⁵N chemical shifts: 67
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_4
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 82, Sequence coverage: 90.2%
- Total number of restraints: 566
- Weight of restraints: 1.0 (566)
- Potential type of restraints: square-well-parabolic (566)
- Range of distance target values: 2.5, 4.9 (Å)
- Histogram of distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 82, Sequence coverage: 87.8%
- Total number of restraints: 128
- Total number of restraints per polymer type: 128
- Weight of restraints: 1.0 (128)
- Potential type of restraints: square-well-parabolic (128)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords Drosha