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BMRB: 25929

2NA6

Transmembrane domain of mouse Fas/CD95 death receptor

Authors

Fu, T., Fu, Q., Chou, J.J., Wu, H.

Assembly

Transmembrane domain of mouse Fas/CD95 death receptor

Entity

1. Transmembrane domain of mouse Fas/CD95 death receptor (polymer, Thiol state: not present), 28 monomers, 3572.428 × 3 Da Detail

RNRLWLLTIL VLLIPLVFIY RKYRKRKS

Total weight

10717.284 Da

Max. entity weight

3572.428 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 28.1 %, Completeness (bb): 62.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	28.1 % (112 of 398)	14.7 % (31 of 211)	35.4 % (56 of 158)	86.2 % (25 of 29)
Backbone	62.7 % (104 of 166)	47.3 % (26 of 55)	63.1 % (53 of 84)	92.6 % (25 of 27)
Sidechain	3.5 % (9 of 260)	3.2 % (5 of 156)	3.9 % (4 of 102)	0.0 % (0 of 2)
Aromatic	0.0 % (0 of 38)	0.0 % (0 of 19)	0.0 % (0 of 18)	0.0 % (0 of 1)
Methyl	8.0 % (4 of 50)	8.0 % (2 of 25)	8.0 % (2 of 25)

1. Mouse Fas Transmembrane Domain

RNRLWLLTIL VLLIPLVFIY RKYRKRKS

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8 (±0.1), Details 15N, 13C doubled labeled sample

#	Name	Isotope labeling	Concentration
1	Mouse Fas Transmembrane Domain	[U-100% 13C; U-100% 15N]	1 (±0.05) mM
2	DMPC	[U-100% 2H] acyl chains	60 (±1.0) mM
3	DHPC	[U-100% 2H] acyl chains	120 (±5.0) mM
4	sodium phosphate	natural abundance	20 (±1.0) mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8 (±0.1), Details 15N, 13C, 2H triple labeled sample

#	Name	Isotope labeling	Concentration
7	Mouse Fas Transmembrane Domain	[U-100% 13C; U-100% 15N; U-85% 2H]	1 (±0.05) mM
8	DMPC	natural abundance	60 (±1.0) mM
9	DHPC	natural abundance	120 (±5.0) mM
10	sodium phosphate	natural abundance	20 (±1.0) mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8 (±0.1), Details Mixed isotope labeled

#	Name	Isotope labeling	Concentration
13	Mouse Fas Transmembrane Domain	[U-100% 15N; U-100% 2H]	0.5 (±0.05) mM
14	Mouse Fas Transmembrane Domain	[U-15% 13C]	0.5 (±0.05) mM
15	DMPC	[U-100% 2H] acyl chains	60 (±1.0) mM
16	DHPC	[U-100% 2H] acyl chains	120 (±5.0) mM
17	sodium phosphate	natural abundance	20 (±1.0) mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %

Protein Blocks Logo

Calculated from 15 models in PDB: 2NA6, Strand ID: A, B, C Detail

Release date

2016-01-24

Citation

Structural Basis and Functional Role of Intramembrane Trimerization of the Fas/CD95 Death Receptor
Fu, Q., Fu, T., Cruz, A.C., Sengupta, P., Thomas, S.K., Wang, S., Siegel, R.M., Wu, H., Chou, J.J.
Mol. Cell (2016), 61, 602-613, PubMed 26853147 , DOI 10.1016/j.molcel.2016.01.009 , Abstract

Related entities 1. Transmembrane domain of mouse Fas/CD95 death receptor, : 1 : 2 : 3 entities Detail

Interaction partners 1. Transmembrane domain of mouse Fas/CD95 death receptor, : 9 interactors Detail

More details for 9 interactors identified by 5 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N TROSY HSQC