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BMRB: 25945

5FRG

The NMR Structure of the Cdc42-interacting region of TOCA1

Authors

Mott, H.R., Watson, J.R., Nietlispach, D., Owen, D.

Assembly

Toca1

Entity

1. Toca1 (polymer, Thiol state: not present), 104 monomers, 12089.51 Da Detail

GPLGSHMKGP ALEDFSHLPP EQRRKRLQQR IDELSRELQK EMDQKDALNK MKDVYEKNPQ MGDPSSLHPK IAETTSNIER LRMEIHKNEA WLSEVEGKVS QRSE

Formula weight

12089.51 Da

Source organism

Xenopus tropicalis

Exptl. method

solution NMR

Refine. method

ARIA2.3; CNS 1.2

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.1 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.1 % (1227 of 1251)	98.2 % (661 of 673)	97.9 % (459 of 469)	98.2 % (107 of 109)
Backbone	97.4 % (594 of 610)	98.1 % (202 of 206)	96.7 % (297 of 307)	97.9 % (95 of 97)
Sidechain	98.9 % (732 of 740)	98.3 % (459 of 467)	100.0 % (261 of 261)	100.0 % (12 of 12)
Aromatic	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (22 of 22)	100.0 % (1 of 1)
Methyl	98.8 % (79 of 80)	97.5 % (39 of 40)	100.0 % (40 of 40)

1. Toca1

GPLGSHMKGP ALEDFSHLPP EQRRKRLQQR IDELSRELQK EMDQKDALNK MKDVYEKNPQ MGDPSSLHPK IAETTSNIER LRMEIHKNEA WLSEVEGKVS QRSE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.500

#	Name	Isotope labeling	Type	Concentration
1	Toca1	[U-13C; U-15N]		900.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 35 models in PDB: 5FRG, Strand ID: A Detail

Release date

2016-05-22

Citation

(1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1
Watson, J.R., Nietlispach, D., Owen, D., Mott, H.R.
Biomol. NMR Assign. (2016), 10, 407-411, PubMed 26988723 , DOI 10.1007/s12104-016-9677-8 , Abstract

Related entities 1. Toca1, : 1 : 2 : 33 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC/HMQC