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BMRB: 25955

5FRF

Solution structure of reduced and zinc-bound RsrA

Authors

Zdanowski, K., Pecqueur, L., Werner, J., Potts, J.ROBYN., Kleanthous, C.

Assembly

Solution structure of reduced and zinc-bound RsrA

Entity

1. ANTI-SIGMA FACTOR RSRA (polymer, Thiol state: all free), 108 monomers, 11817.92 Da Detail

GSHMSAGEPH ETDCSEILDH LYEFLDKEMP DSDAVKFEHH FEESSPCLEK YGLEQAVKKL VKRAAGQDDV PGDLRAKVMG RLDLIRSGQS VPEHDVAAAP SSSAPQES

2. Solution structure of reduced and zinc-bound RsrA, entity ZN (non-polymer), 65.409 Da

Total weight

11883.329 Da

Max. entity weight

11817.92 Da

Source organism

Streptomyces coelicolor

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 76.3 %, Completeness (bb): 91.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.3 % (909 of 1192)	83.3 % (518 of 622)	63.7 % (296 of 465)	90.5 % (95 of 105)
Backbone	91.8 % (582 of 634)	93.1 % (201 of 216)	91.2 % (289 of 317)	91.1 % (92 of 101)
Sidechain	63.3 % (417 of 659)	78.1 % (317 of 406)	39.0 % (97 of 249)	75.0 % (3 of 4)
Aromatic	42.9 % (30 of 70)	85.7 % (30 of 35)	0.0 % (0 of 35)
Methyl	60.6 % (57 of 94)	95.7 % (45 of 47)	25.5 % (12 of 47)

1. ANTI-SIGMA FACTOR RSRA

GSHMSAGEPH ETDCSEILDH LYEFLDKEMP DSDAVKFEHH FEESSPCLEK YGLEQAVKKL VKRAAGQDDV PGDLRAKVMG RLDLIRSGQS VPEHDVAAAP SSSAPQES

Show sample condition

Sample

Solvent system 5% D2O/(5% water, Pressure 1 atm, Temperature 298.000 K, pH 7.100, Details 1-1.5mM

#	Name	Isotope labeling	Type	Concentration
1	ANTI-SIGMA FACTOR RSRA	[U-13C; U-15N]		1.25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 5FRF, Strand ID: A Detail

Release date

2016-07-31

Citation

The anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic core
Rajasekar, K.V., Zdanowski, K., Yan, J., Hopper, J.T.S., Francis, M.R., Seepersad, C., Sharp, C., Pecqueur, L., Werner, J., Robinson, C.V., Mohammed, S., Potts, J.ROBYN., Kleanthous, C.
Nat. Commun. (2016), 7, 12194-12194, PubMed 27432510 , DOI 10.1038/ncomms12194 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25956 released on 2016-07-31
Title Solution structure of oxidised RsrA and without zinc ion

Related entities 1. ANTI-SIGMA FACTOR RSRA, : 1 : 1 entities Detail

Experiments performed 9 experiments Detail

1 NOESY