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BMRB: 25956

5FRH

Solution structure of oxidised RsrA and without zinc ion

Authors

Zdanowski, K., Pecqueur, L., Werner, J., Potts, J.ROBYN., Kleanthous, C.

Assembly

Solution structure of oxidised and zinc-bound RsrA

Entity

1. Solution structure of oxidised and zinc-bound RsrA (polymer, Thiol state: all disulfide bound), 108 monomers, 11817.92 Da Detail

GSHMSAGEPH ETDCSEILDH LYEFLDKEMP DSDAVKFEHH FEESSPCLEK YGLEQAVKKL VKRAAGQDDV PGDLRAKVMG RLDLIRSGQS VPEHDVAAAP SSSAPQES

Formula weight

11817.92 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS14:SG	1:CYS47:SG

Source organism

Streptomyces coelicolor

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 85.2 %, Completeness (bb): 94.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.2 % (1016 of 1192)	93.1 % (579 of 622)	73.5 % (342 of 465)	90.5 % (95 of 105)
Backbone	94.8 % (601 of 634)	94.4 % (204 of 216)	95.3 % (302 of 317)	94.1 % (95 of 101)
Sidechain	77.7 % (512 of 659)	92.4 % (375 of 406)	55.0 % (137 of 249)	0.0 % (0 of 4)
Aromatic	42.9 % (30 of 70)	85.7 % (30 of 35)	0.0 % (0 of 35)
Methyl	75.5 % (71 of 94)	97.9 % (46 of 47)	53.2 % (25 of 47)

1. ANTI-SIGMA FACTOR RSRA

GSHMSAGEPH ETDCSEILDH LYEFLDKEMP DSDAVKFEHH FEESSPCLEK YGLEQAVKKL VKRAAGQDDV PGDLRAKVMG RLDLIRSGQS VPEHDVAAAP SSSAPQES

Show sample condition

Sample

Solvent system 5% D2O/(5% water, Pressure 1 atm, Temperature 298.000 K, pH 7.100, Details 1-1.5mM

#	Name	Isotope labeling	Type	Concentration
1	ANTI-SIGMA FACTOR RSRA	[U-13C; U-15N]		1.25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 5FRH, Strand ID: A Detail

Release date

2016-07-31

Citation

The anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic core
Rajasekar, K.V., Zdanowski, K., Yan, J., Hopper, J.T.S., Francis, M.R., Seepersad, C., Sharp, C., Pecqueur, L., Werner, J., Robinson, C.V., Mohammed, S., Potts, J.ROBYN., Kleanthous, C.
Nat. Commun. (2016), 7, 12194-12194, PubMed 27432510 , DOI 10.1038/ncomms12194 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25955 released on 2016-07-31
Title Solution structure of reduced and zinc-bound RsrA

Related entities 1. Solution structure of oxidised and zinc-bound RsrA, : 1 : 1 entities Detail

Experiments performed 9 experiments Detail

1 NOESY