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NMR structure of the Acidic domain of SYNCRIP (24-140)
Authors
Serrano, P., Wuthrich, K., Beuck, C.
Assembly
Acidic domain of SYNCRIP (24-140)
Entity
1. Acidic domain of SYNCRIP (24-140) (polymer), 118 monomers, 12915.33 Da Detail

GSENFQTLLD AGLPQKVAEK LDEIYVAGLV AHSDLDERAI EALKEFNEDG ALAVLQQFKD SDLSHVQNKS AFLCGVMKTY RQREKQGTKV ADSSKGPDEA KIKALLERTG YTLDVTTG


Formula weight
12915.33 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 92.9 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All92.9 % (1245 of 1340)91.9 % (639 of 695)92.5 % (480 of 519)100.0 % (126 of 126)
Backbone96.0 % (676 of 704)97.1 % (236 of 243)93.9 % (324 of 345)100.0 % (116 of 116)
Sidechain90.9 % (677 of 745)89.2 % (403 of 452)93.3 % (264 of 283)100.0 % (10 of 10)
Aromatic80.6 % (58 of 72)83.3 % (30 of 36)77.8 % (28 of 36)
Methyl97.1 % (134 of 138)95.7 % (66 of 69)98.6 % (68 of 69)

1. entity

GSENFQTLLD AGLPQKVAEK LDEIYVAGLV AHSDLDERAI EALKEFNEDG ALAVLQQFKD SDLSHVQNKS AFLCGVMKTY RQREKQGTKV ADSSKGPDEA KIKALLERTG YTLDVTTG

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0


#NameIsotope labelingTypeConcentration
1entity[U-99% 13C; U-98% 15N]1.2 mM
2sodium phosphatenatural abundance20 mM
3sodium chloridenatural abundance50 mM
4sodium azidenatural abundance5 mM

LACS Plot; CA
Referencing offset: 2.73 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 2.73 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.13 ppm, Outliers: 3 Detail
LACS Plot; CO
Referencing offset: 2.63 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 19 models in PDB: 2NBB, Strand ID: A Detail


Release date
2016-05-30
Citation
The acidic domain is a unique structural feature of the splicing factor SYNCRIP
Beuck, C., Williamson, J.R., Wuthrich, K., Serrano, P.
Protein Sci. (2016), 25, 1545-1550, PubMed 27081926 , DOI 10.1002/pro.2935 ,
Related entities 1. Acidic domain of SYNCRIP (24-140), : 1 : 4 : 14 entities Detail
Interaction partners 1. Acidic domain of SYNCRIP (24-140), : 16 interactors Detail
Experiments performed 7 experiments Detail
NMR combined restraints 3 contents Detail
Keywords Acidic domain, SYNCRIP