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BMRB: 25976

2NBH

Solution structure of the HYD1 hydrophobin from Schizophyllum commune

Authors

Langelaan, D., Smith, S., Grondin, J.

Assembly

fungal hydrophobin

Entity

1. fungal hydrophobin (polymer, Thiol state: all disulfide bound), 107 monomers, 10863.38 Da Detail

KAMADIGSTA VPRDVNGGTP PKSCSSGPVY CCNKTEDSKH LDKGTTALLG LLNIKIGDLK DLVGLNCSPL SVIGVGGNSC SAQTVCCTNT YQHGLVNVGC TPINIGL

Formula weight

10863.38 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS24:SG	1:CYS86:SG
2	disulfide	sing	1:CYS31:SG	1:CYS80:SG
3	disulfide	sing	1:CYS32:SG	1:CYS67:SG
4	disulfide	sing	1:CYS87:SG	1:CYS100:SG

Source organism

Schizophyllum commune

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 85.3 %, Completeness (bb): 87.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.3 % (958 of 1123)	81.9 % (471 of 575)	89.2 % (390 of 437)	87.4 % (97 of 111)
Backbone	87.9 % (554 of 630)	86.9 % (193 of 222)	88.6 % (272 of 307)	88.1 % (89 of 101)
Sidechain	83.1 % (487 of 586)	78.8 % (278 of 353)	90.1 % (201 of 223)	80.0 % (8 of 10)
Aromatic	83.3 % (20 of 24)	83.3 % (10 of 12)	83.3 % (10 of 12)
Methyl	92.4 % (122 of 132)	92.4 % (61 of 66)	92.4 % (61 of 66)

1. Hydrophobin

KAMADIGSTA VPRDVNGGTP PKSCSSGPVY CCNKTEDSKH LDKGTTALLG LLNIKIGDLK DLVGLNCSPL SVIGVGGNSC SAQTVCCTNT YQHGLVNVGC TPINIGL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	Hydrophobin	[U-13C; U-15N]	protein	1 mM
2	TRIS	natural abundance	buffer	25 mM
3	sodium chloride	natural abundance	salt	150 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	[U-2H]	solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.33 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2NBH, Strand ID: A Detail

Release date

2016-03-29

Citation

Structure of a fungal hydrophobin
Langelaan, D., Smith, S., Master, E., Gandier, J., Grondin, J.
Not known

Related entities 1. fungal hydrophobin, : 1 : 2 : 9 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC