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BMRB: 25993

2NBR

The Solution Structure of Human gammaC-crystallin

Authors

Dixit, K., Pande, A., Pande, J., Sarma, S.P.

Assembly

Human gammaC-crystallin

Entity

1. Human gammaC-crystallin (polymer, Thiol state: all free), 173 monomers, 20747.25 Da Detail

GKITFYEDRA FQGRSYETTT DCPNLQPYFS RCNSIRVESG CWMLYERPNY QGQQYLLRRG EYPDYQQWMG LSDSIRSCCL IPQTVSHRLR LYEREDHKGL MMELSEDCPS IQDRFHLSEI RSLHVLEGCW VLYELPNYRG RQYLLRPQEY RRCQDWGAMD AKAGSLRRVV DLY

Formula weight

20747.25 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, coupling_constants, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.5 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.5 % (1728 of 2094)	80.7 % (892 of 1105)	81.8 % (658 of 804)	96.2 % (178 of 185)
Backbone	97.3 % (994 of 1022)	97.1 % (339 of 349)	97.0 % (493 of 508)	98.2 % (162 of 165)
Sidechain	72.5 % (895 of 1234)	73.1 % (553 of 756)	71.2 % (326 of 458)	80.0 % (16 of 20)
Aromatic	33.3 % (72 of 216)	55.6 % (60 of 108)	7.7 % (8 of 104)	100.0 % (4 of 4)
Methyl	81.0 % (115 of 142)	84.5 % (60 of 71)	77.5 % (55 of 71)

1. Molecule 1

GKITFYEDRA FQGRSYETTT DCPNLQPYFS RCNSIRVESG CWMLYERPNY QGQQYLLRRG EYPDYQQWMG LSDSIRSCCL IPQTVSHRLR LYEREDHKGL MMELSEDCPS IQDRFHLSEI RSLHVLEGCW VLYELPNYRG RQYLLRPQEY RRCQDWGAMD AKAGSLRRVV DLY

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Molecule 1	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	potassium phosphate	natural abundance	buffer	20 mM
3	DTT	natural abundance		5 mM
4	sodium chloride	natural abundance	salt	20 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 1.11 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2NBR, Strand ID: A Detail

Coupling constant

123 J values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 7 Detail

Release date

2016-05-31

Citation

Nuclear Magnetic Resonance Structure of a Major Lens Protein, Human γC-Crystallin: Role of the Dipole Moment in Protein Solubility
Dixit, K., Pande, A., Pande, J., Sarma, S.
Biochemistry (2016), 55, 3136-3149, PubMed 27187112 , DOI 10.1021/acs.biochem.6b00359 , Abstract

Related entities 1. Human gammaC-crystallin, : 1 : 2 : 308 entities Detail

Interaction partners 1. Human gammaC-crystallin, : 4 interactors Detail

More details for 4 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC/HMQC