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BMRB: 25995

2NBV

Protein complex

Authors

Chen, X., Walters, K.J.

Assembly

human Rpn13 Pru domain

Entity

1. human Rpn13 Pru domain, entity 1 (polymer, Thiol state: all free), 150 monomers, 16718.84 Da Detail

MTTSGALFPS LVPGSRGASN KYLVEFRAGK MSLKGTTVTP DKRKGLVYIQ QTDDSLIHFC WKDRTSGNVE DDLIIFPDDC EFKRVPQCPS GRVYVLKFKA GSKRLFFWMQ EPKTDQDEEH CRKVNEYLNN PPMPGALGAS GSSGHELSAL

2. human Rpn13 Pru domain, entity 2 (polymer, Thiol state: all free), 78 monomers, 8749.065 Da Detail

APAEPKIIKV TVKTPKEKEE FAVPENSSVQ QFKEAISKRF KSQTDQLVLI FAGKILKDQD TLIQHGIHDG LTVHLVIK

Total weight

25467.906 Da

Max. entity weight

16718.84 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 65.8 %, Completeness: 56.5 %, Completeness (bb): 64.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	56.5 % (1524 of 2699)	55.8 % (789 of 1414)	55.6 % (585 of 1052)	64.4 % (150 of 233)
Backbone	64.9 % (869 of 1340)	64.4 % (295 of 458)	65.1 % (435 of 668)	65.0 % (139 of 214)
Sidechain	50.4 % (792 of 1571)	51.7 % (494 of 956)	48.2 % (287 of 596)	57.9 % (11 of 19)
Aromatic	4.5 % (9 of 200)	7.0 % (7 of 100)	0.0 % (0 of 98)	100.0 % (2 of 2)
Methyl	54.6 % (130 of 238)	54.6 % (65 of 119)	54.6 % (65 of 119)

1. entity 1

MTTSGALFPS LVPGSRGASN KYLVEFRAGK MSLKGTTVTP DKRKGLVYIQ QTDDSLIHFC WKDRTSGNVE DDLIIFPDDC EFKRVPQCPS GRVYVLKFKA GSKRLFFWMQ EPKTDQDEEH CRKVNEYLNN PPMPGALGAS GSSGHELSAL

2. entity 2

APAEPKIIKV TVKTPKEKEE FAVPENSSVQ QFKEAISKRF KSQTDQLVLI FAGKILKDQD TLIQHGIHDG LTVHLVIK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N; U-70% 2H]	protein	0.7 mM
2	entity_2	natural abundance	protein	0.7 mM
3	sodium phosphate	natural abundance	buffer	20 mM
4	sodium chloride	natural abundance	salt	50 mM
5	DTT	natural abundance		2 mM
6	sodium azide	natural abundance		0.1 %
7	H2O	natural abundance	solvent	95 %
8	D2O	[U-2H]	solvent	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
9	entity_1	[U-100% 15N]	protein	0.7 mM
10	entity_2	natural abundance	protein	0.7 mM
11	sodium phosphate	natural abundance	buffer	20 mM
12	sodium chloride	natural abundance	salt	50 mM
13	DTT	natural abundance		2 mM
14	sodium azide	natural abundance		0.1 %
15	H2O	natural abundance	solvent	95 %
16	D2O	[U-2H]	solvent	5 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
17	entity_1	[U-100% 13C]	protein	0.7 mM
18	entity_2	natural abundance	protein	0.7 mM
19	sodium phosphate	natural abundance	buffer	20 mM
20	sodium chloride	natural abundance	salt	50 mM
21	DTT	natural abundance		2 mM
22	sodium azide	natural abundance		0.1 %
23	D2O	[U-2H]	solvent	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.31 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.31 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 3.1 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2NBV, Strand ID: A, B Detail

Release date

2016-07-17

Citation

Structures of Rpn1 T1:Rad23 and hRpn13:hPLIC2 Reveal Distinct Binding Mechanisms between Substrate Receptors and Shuttle Factors of the Proteasome
Chen, X., Randles, L., Shi, K., Tarasov, S.G., Aihara, H., Walters, K.J.
Structure (2016), 24, 1257-1270, PubMed 27396824 , DOI 10.1016/j.str.2016.05.018 , Abstract

Related entities 1. human Rpn13 Pru domain, entity 1, : 1 : 7 : 2 : 18 entities Detail

Related entities 2. human Rpn13 Pru domain, entity 2, : 1 : 3 : 84 entities Detail

Interaction partners 2. human Rpn13 Pru domain, entity 2, : 20 interactors Detail

More details for 20 interactors identified by 12 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC