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Found 1 Document (2.022 seconds)

BMRB: 26041

2NCZ

Solution NMR structures of BRD4 ET domain in complex with NSD3_1 peptide

Authors

Zeng, L., ZHOU, M.

Assembly

BRD4 ET with NSD3 1

Entity

1. BRD4 ET with NSD3 1, entity 1 (polymer, Thiol state: all free), 83 monomers, 9726.987 Da Detail

SEEEDKCKPM SYEEKRQLSL DINKLPGEKL GRVVHIIQSR EPSLKNSNPD EIEIDFETLK PSTLRELERY VTSCLRKKRK PQA

2. BRD4 ET with NSD3 1, entity 2 (polymer, Thiol state: all free), 12 monomers, 1428.714 Da Detail

EIKLKITKTI QN

Total weight

11155.701 Da

Max. entity weight

9726.987 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.9 %, Completeness (bb): 80.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.9 % (987 of 1177)	89.3 % (565 of 633)	76.3 % (341 of 447)	83.5 % (81 of 97)
Backbone	80.6 % (450 of 558)	97.8 % (182 of 186)	67.8 % (192 of 283)	85.4 % (76 of 89)
Sidechain	88.1 % (627 of 712)	85.7 % (383 of 447)	93.0 % (239 of 257)	62.5 % (5 of 8)
Aromatic	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (15 of 15)
Methyl	93.8 % (90 of 96)	97.9 % (47 of 48)	89.6 % (43 of 48)

1. entity 1

SEEEDKCKPM SYEEKRQLSL DINKLPGEKL GRVVHIIQSR EPSLKNSNPD EIEIDFETLK PSTLRELERY VTSCLRKKRK PQA

2. entity 2

EIKLKITKTI QN

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance	protein	0.0 ~ 0.0 mM
2	entity_2	natural abundance	protein	0.0 ~ 0.0 mM
3	sodium phosphate	natural abundance	buffer	10 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-100% 2H]		2 mM
6	D2O	[U-2H]	solvent	100 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	entity_1	natural abundance	protein	0.0 ~ 0.0 mM
8	entity_2	natural abundance	protein	0.0 ~ 0.0 mM
9	sodium phosphate	natural abundance	buffer	10 mM
10	sodium chloride	natural abundance	salt	100 mM
11	DTT	[U-100% 2H]		2 mM
12	H2O	natural abundance	solvent	90 %
13	D2O	[U-2H]	solvent	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2NCZ, Strand ID: A, B Detail

Release date

2016-06-29

Citation

Structural Mechanism of Transcriptional Regulator NSD3 Recognition by the ET Domain of BRD4
Zhang, Q., Zeng, L., Shen, C., Ju, Y., Konuma, T., Zhao, C., Vakoc, C.R., ZHOU, M.
Structure (2016), 24, 1201-1208, PubMed 27291650 , DOI 10.1016/j.str.2016.04.019 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 26042 released on 2016-06-29
    Title Solution NMR structures of BRD4 ET domain with LANA peptide
  BMRB: 26043 released on 2016-06-29
    Title Solution NMR structures of BRD4 ET domain in complex with NSD3_3 peptide

Related entities 1. BRD4 ET with NSD3 1, entity 1, : 5 : 80 entities Detail

Related entities 2. BRD4 ET with NSD3 1, entity 2, : 3 entities Detail

Interaction partners 1. BRD4 ET with NSD3 1, entity 1, : 42 interactors Detail

More details for 42 interactors identified by 19 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	entity_1	natural abundance	protein	0.0 ~ 0.0 mM
8	entity_2	natural abundance	protein	0.0 ~ 0.0 mM
9	sodium phosphate	natural abundance	buffer	10 mM
10	sodium chloride	natural abundance	salt	100 mM
11	DTT	[U-100% 2H]		2 mM
12	H2O	natural abundance	solvent	90 %
13	D2O	[U-2H]	solvent	10 %

2 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	entity_1	natural abundance	protein	0.0 ~ 0.0 mM
8	entity_2	natural abundance	protein	0.0 ~ 0.0 mM
9	sodium phosphate	natural abundance	buffer	10 mM
10	sodium chloride	natural abundance	salt	100 mM
11	DTT	[U-100% 2H]		2 mM
12	H2O	natural abundance	solvent	90 %
13	D2O	[U-2H]	solvent	10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	entity_1	natural abundance	protein	0.0 ~ 0.0 mM
8	entity_2	natural abundance	protein	0.0 ~ 0.0 mM
9	sodium phosphate	natural abundance	buffer	10 mM
10	sodium chloride	natural abundance	salt	100 mM
11	DTT	[U-100% 2H]		2 mM
12	H2O	natural abundance	solvent	90 %
13	D2O	[U-2H]	solvent	10 %

4 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	entity_1	natural abundance	protein	0.0 ~ 0.0 mM
8	entity_2	natural abundance	protein	0.0 ~ 0.0 mM
9	sodium phosphate	natural abundance	buffer	10 mM
10	sodium chloride	natural abundance	salt	100 mM
11	DTT	[U-100% 2H]		2 mM
12	H2O	natural abundance	solvent	90 %
13	D2O	[U-2H]	solvent	10 %

5 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance	protein	0.0 ~ 0.0 mM
2	entity_2	natural abundance	protein	0.0 ~ 0.0 mM
3	sodium phosphate	natural abundance	buffer	10 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-100% 2H]		2 mM
6	D2O	[U-2H]	solvent	100 %

6 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance	protein	0.0 ~ 0.0 mM
2	entity_2	natural abundance	protein	0.0 ~ 0.0 mM
3	sodium phosphate	natural abundance	buffer	10 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-100% 2H]		2 mM
6	D2O	[U-2H]	solvent	100 %

7 3D filtered 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance	protein	0.0 ~ 0.0 mM
2	entity_2	natural abundance	protein	0.0 ~ 0.0 mM
3	sodium phosphate	natural abundance	buffer	10 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-100% 2H]		2 mM
6	D2O	[U-2H]	solvent	100 %

8 3D filtered 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance	protein	0.0 ~ 0.0 mM
2	entity_2	natural abundance	protein	0.0 ~ 0.0 mM
3	sodium phosphate	natural abundance	buffer	10 mM
4	sodium chloride	natural abundance	salt	100 mM
5	DTT	[U-100% 2H]		2 mM
6	D2O	[U-2H]	solvent	100 %

nullKeywords BET, BRD4, ET, LANA, NSD3

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Found 1 Document (2.022 seconds)

BMRB: 26041

Sample #1

Sample #2

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. BRD4 ET with NSD3 1, entity 1, : 5 : 80 entities Detail

Related entities 2. BRD4 ET with NSD3 1, entity 2, : 3 entities Detail

Interaction partners 1. BRD4 ET with NSD3 1, entity 1, : 42 interactors Detail

Experiments performed 8 experiments Detail

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