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BMRB: 26052

2ND9

Solution structure of MapZ extracellular domain first subdomain

Authors

Jean, N.L., Manuse, S., Guinot, M., Bougault, C.M., Grangeasse, C., Simorre, J.

Assembly

MapZ QG

Entity

1. MapZ QG (polymer, Thiol state: not present), 135 monomers, 14847.31 Da Detail

GHMQVARSTK EIETSQSTTA NQSDVDDFNT LYDAFYTNSN KTALKNSQFD KLSQLKTLLD KLEGSREHTL AKSKYDSLAT QIKAIQDVNA QFEKPAIVDG VLDTNAKAKS DAKFTDIKTG NTELDKVLDK AISLG

Formula weight

14847.31 Da

Source organism

Streptococcus pneumoniae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 91.2 %, Completeness (bb): 94.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.2 % (1406 of 1542)	88.3 % (705 of 798)	94.4 % (561 of 594)	93.3 % (140 of 150)
Backbone	94.2 % (761 of 808)	91.6 % (251 of 274)	96.0 % (384 of 400)	94.0 % (126 of 134)
Sidechain	89.2 % (771 of 864)	86.6 % (454 of 524)	93.5 % (303 of 324)	87.5 % (14 of 16)
Aromatic	95.1 % (78 of 82)	95.1 % (39 of 41)	95.1 % (39 of 41)
Methyl	96.8 % (149 of 154)	96.1 % (74 of 77)	97.4 % (75 of 77)

1. MapZ QG

GHMQVARSTK EIETSQSTTA NQSDVDDFNT LYDAFYTNSN KTALKNSQFD KLSQLKTLLD KLEGSREHTL AKSKYDSLAT QIKAIQDVNA QFEKPAIVDG VLDTNAKAKS DAKFTDIKTG NTELDKVLDK AISLG

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	MapZ_extra1	[U-100% 13C; U-100% 15N]	2.0 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2ND9, Strand ID: A Detail

Release date

2016-06-26

Citation

Structure-function analysis of the extracellular domain of the pneumococcal cell division site positioning protein MapZ
Manuse, S., Jean, N.L., Guinot, M., Lavergne, J., Laguri, C., Bougault, C.M., Van Nieuwenhze, M.S., Grangeasse, C., Simorre, J.
Nat. Commun. (2016), 7, 12071-12071, PubMed 27346279 , DOI 10.1038/ncomms12071 , Abstract

Related entities 1. MapZ QG, : 1 : 1 entities Detail

Interaction partners 1. MapZ QG, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC/HMQC