BMRB: 26068

Structure of DNA-binding HU protein from micoplasma Mycoplasma gallisepticum

Authors

Altukhov, D.A., Talyzina, A.A., Agapova, Y.K., Vlaskina, A.V., Korzhenevskiy, D.A., Bocharov, E.V., Rakitina, T.V., Timofeev, V.I., Popov, V.O.

Assembly

DNA-binding HU protein

Entity

1. DNA-binding HU protein (polymer, Thiol state: not present), 99 monomers, 10961.91 × 2 Da Detail

MAKIKSLSAA EYLKEMADET NIKVQDIRLV VTSLQKVLAK ELATTGEVRL FDIGKFKLVT TKPRTGINPK TKQKIQIPAG KKIKLTVSKI LTDAVDSHK

Total weight

21923.82 Da

Max. entity weight

10961.91 Da

Source organism

Mycoplasma gallisepticum

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 42.8 %, Completeness (bb): 85.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	42.8 % (512 of 1197)	26.5 % (166 of 627)	53.8 % (252 of 468)	92.2 % (94 of 102)
Backbone	85.0 % (500 of 588)	78.9 % (157 of 199)	85.7 % (251 of 293)	95.8 % (92 of 96)
Sidechain	11.1 % (78 of 704)	2.1 % (9 of 428)	24.8 % (67 of 270)	33.3 % (2 of 6)
Aromatic	0.0 % (0 of 32)	0.0 % (0 of 16)	0.0 % (0 of 16)
Methyl	4.2 % (6 of 144)	0.0 % (0 of 72)	8.3 % (6 of 72)

1. entity

MAKIKSLSAA EYLKEMADET NIKVQDIRLV VTSLQKVLAK ELATTGEVRL FDIGKFKLVT TKPRTGINPK TKQKIQIPAG KKIKLTVSKI LTDAVDSHK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-13C; U-15N]		1 mM
2	D2O	[U-2H]		5 %
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 mM

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LACS Plot; CA

Referencing offset: 0.69 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.69 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.84 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 15 models in PDB: 2NDP, Strand ID: A, B Detail

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Release date

2016-11-02

Citation

Structure of DNA-binding HU protein from micoplasma Mycoplasma gallisepticum
Altukhov, D.A., Talyzina, A.A., Agapova, Y.K., Vlaskina, A.V., Korzhenevskiy, D.A., Bocharov, E.V., Rakitina, T.V., Timofeev, V.I., Popov, V.O.
Biochem. Biophys. Res. Commun.

Related entities 1. DNA-binding HU protein, : 1 : 75 entities Detail

Experiments performed 2 experiments Detail