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BMRB: 26517

5AGQ

TAM DOMAIN OF TIP5_BAZ2A

Authors

Anosova, I., Melnik, S., Tripsianes, K., Kateb, F., Grummt, I., Sattler, M.

Assembly

TAM DOMAIN OF TIP5 BAZ2A

Entity

1. TAM DOMAIN OF TIP5 BAZ2A (polymer, Thiol state: all free), 109 monomers, 13004.66 Da Detail

RRRIATPEEV RLPLQHGWRR EVRIKKGSHR WQGETWYYGP CGKRMKQFPE VIKYLSRNVV HSVRREHFSF SPRMPVGDFF EERDTPEGLQ WVQLSAEEIP SRIQAITGS

Formula weight

13004.66 Da

Source organism

Exptl. method

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.7 %, Completeness (bb): 80.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.7 % (1197 of 1349)	96.1 % (690 of 718)	76.9 % (399 of 519)	96.4 % (108 of 112)
Backbone	80.6 % (514 of 638)	97.7 % (213 of 218)	63.9 % (204 of 319)	96.0 % (97 of 101)
Sidechain	96.2 % (781 of 812)	95.4 % (477 of 500)	97.3 % (293 of 301)	100.0 % (11 of 11)
Aromatic	100.0 % (138 of 138)	100.0 % (69 of 69)	100.0 % (65 of 65)	100.0 % (4 of 4)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. entity 1

RRRIATPEEV RLPLQHGWRR EVRIKKGSHR WQGETWYYGP CGKRMKQFPE VIKYLSRNVV HSVRREHFSF SPRMPVGDFF EERDTPEGLQ WVQLSAEEIP SRIQAITGS

Show sample condition

Sample

Solvent system 20MM SODIUM PHOSPHATE PH 7.0, 50MM NACL, 1MM DTT, 90%H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.000, Details TAM 0.6 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TAM	[U-13C; U-15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	NACL	natural abundance		50 mM
4	DTT	natural abundance		1 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 5AGQ, Strand ID: A Detail

Release date

2015-04-27

Citation

A novel RNA binding surface of the TAM domain of TIP5/BAZ2A mediates epigenetic regulation of rRNA genes
Anosova, I., Melnik, S., Tripsianes, K., Kateb, F., Grummt, I., Sattler, M.
Nucleic Acids Res. (2015), 43, 5208-5220, PubMed 25916849 , DOI 10.1093/nar/gkv365 , Abstract

Related entities 1. TAM DOMAIN OF TIP5 BAZ2A, : 2 : 11 entities Detail

Interaction partners 1. TAM DOMAIN OF TIP5 BAZ2A, : 5 interactors Detail

More details for 5 interactors identified by 4 citations

Experiments performed 9 experiments Detail

1 15N-HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 20MM SODIUM PHOSPHATE PH 7.0, 50MM NACL, 1MM DTT, 90%H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.000, Details TAM 0.6 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TAM	[U-13C; U-15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	NACL	natural abundance		50 mM
4	DTT	natural abundance		1 mM

2 13C-HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 20MM SODIUM PHOSPHATE PH 7.0, 50MM NACL, 1MM DTT, 90%H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.000, Details TAM 0.6 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TAM	[U-13C; U-15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	NACL	natural abundance		50 mM
4	DTT	natural abundance		1 mM

3 HNCACB

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 20MM SODIUM PHOSPHATE PH 7.0, 50MM NACL, 1MM DTT, 90%H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.000, Details TAM 0.6 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TAM	[U-13C; U-15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	NACL	natural abundance		50 mM
4	DTT	natural abundance		1 mM

4 HN(CO)CACB

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 20MM SODIUM PHOSPHATE PH 7.0, 50MM NACL, 1MM DTT, 90%H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.000, Details TAM 0.6 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TAM	[U-13C; U-15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	NACL	natural abundance		50 mM
4	DTT	natural abundance		1 mM

5 CC(CO)NH-TOCSY

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 20MM SODIUM PHOSPHATE PH 7.0, 50MM NACL, 1MM DTT, 90%H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.000, Details TAM 0.6 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TAM	[U-13C; U-15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	NACL	natural abundance		50 mM
4	DTT	natural abundance		1 mM

6 HCCH-TOCSY

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 20MM SODIUM PHOSPHATE PH 7.0, 50MM NACL, 1MM DTT, 90%H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.000, Details TAM 0.6 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TAM	[U-13C; U-15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	NACL	natural abundance		50 mM
4	DTT	natural abundance		1 mM

7 15N-EDITED 3D NOESY

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 20MM SODIUM PHOSPHATE PH 7.0, 50MM NACL, 1MM DTT, 90%H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.000, Details TAM 0.6 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TAM	[U-13C; U-15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	NACL	natural abundance		50 mM
4	DTT	natural abundance		1 mM

8 13C-EDITED 3D NOESY (ALIPHATICS)

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 20MM SODIUM PHOSPHATE PH 7.0, 50MM NACL, 1MM DTT, 90%H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.000, Details TAM 0.6 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TAM	[U-13C; U-15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	NACL	natural abundance		50 mM
4	DTT	natural abundance		1 mM

9 13C-EDITED 3D NOESY (AROMATICS)

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 20MM SODIUM PHOSPHATE PH 7.0, 50MM NACL, 1MM DTT, 90%H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.000, Details TAM 0.6 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TAM	[U-13C; U-15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	NACL	natural abundance		50 mM
4	DTT	natural abundance		1 mM

nullKeywords PROTEIN RNA INTERACTI EPIGENETICS, RRNA GENE SILENCING, TAM DOMAIN, TIP5/BAZ2A, TRANSCRIPTION