NMR solution structure of the putative transfer protein TraH from Gram-positive conjugative plasmid pIP501
SNTNQSESEK IIKEFYKTVY NYEKSQKEIS MTTVKELATD NVYQELQNEI NVNNSYSPQQ NTIQKSSVNE NEIKILAYES KDNSQQYLVT APIHQVFNGT KNDFEINQLI QIKNQKITQR TTIQLGEE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.5 % (1507 of 1561) | 96.2 % (791 of 822) | 97.8 % (569 of 582) | 93.6 % (147 of 157) |
Backbone | 97.4 % (744 of 764) | 96.5 % (247 of 256) | 98.2 % (375 of 382) | 96.8 % (122 of 126) |
Sidechain | 96.1 % (887 of 923) | 96.1 % (544 of 566) | 97.5 % (318 of 326) | 80.6 % (25 of 31) |
Aromatic | 95.6 % (86 of 90) | 97.8 % (44 of 45) | 93.3 % (42 of 45) | |
Methyl | 100.0 % (132 of 132) | 100.0 % (66 of 66) | 100.0 % (66 of 66) |
1. TRAH
SNTNQSESEK IIKEFYKTVY NYEKSQKEIS MTTVKELATD NVYQELQNEI NVNNSYSPQQ NTIQKSSVNE NEIKILAYES KDNSQQYLVT APIHQVFNGT KNDFEINQLI QIKNQKITQR TTIQLGEESolvent system 10% D2O, 90% water, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRAH | [U-13C; U-15N] | 0.5 mM | |
2 | PO4 | natural abundance | 50 mM | |
3 | NaCl | natural abundance | 100 mM |