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BMRB: 26547

4YYP

STIL binding to the Polo-box 3 of PLK4 regulates centriole duplication - Backbone assignment of human Polo-box 3 bound to STIL

Authors

Boehm, R., Arquint, C., Gabryjonczyk, A., Imseng, S., Sauer, E., Nigg, E., Maier, T., Hiller, S.

Assembly

PLK4-PB3/STIL-CC

Entity

1. PLK4-PB3 (polymer, Thiol state: all free), 88 monomers, 9622.769 Da Detail

GSAQLLKSVF VKNVGWATQL TSGAVWVQFN DGSQLVVQAG VSSISYTSPN GQTTRYGENE KLPDYIKQKL QCLSSILLMF SNPTPNFH

2. PLK4-PB3 (polymer, Thiol state: not present), 32 monomers, 3842.383 Da Detail

PDAYRFLTEQ DRQLRLLQAQ IQRLLEAQSL MP

Total weight

13465.151 Da

Max. entity weight

9622.769 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 67.5 %, Completeness: 23.0 %, Completeness (bb): 43.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	23.0 % (328 of 1428)	12.1 % (90 of 744)	30.3 % (166 of 548)	52.9 % (72 of 136)
Backbone	43.4 % (307 of 708)	32.0 % (77 of 241)	45.0 % (159 of 353)	62.3 % (71 of 114)
Sidechain	11.4 % (95 of 833)	2.8 % (14 of 503)	26.0 % (80 of 308)	4.5 % (1 of 22)
Aromatic	5.5 % (6 of 110)	3.6 % (2 of 55)	7.5 % (4 of 53)	0.0 % (0 of 2)
Methyl	6.4 % (9 of 140)	4.3 % (3 of 70)	8.6 % (6 of 70)

1. PLK4-PB3

GSAQLLKSVF VKNVGWATQL TSGAVWVQFN DGSQLVVQAG VSSISYTSPN GQTTRYGENE KLPDYIKQKL QCLSSILLMF SNPTPNFH

2. PLK4-PB3

PDAYRFLTEQ DRQLRLLQAQ IQRLLEAQSL MP

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293.15 K, pH 7

#	Name	Isotope labeling	Concentration
1	PLK4-PB3	[U-13C; U-15N; U-2H]	800 uM
2	SCL/TAL1 INTERRUPTING LOCUS PROTEIN-COILED COIL REGION (STIL-CC)	natural abundance	800 uM
3	sodium chloride	natural abundance	30 mM
4	TCEP	natural abundance	2 mM
5	MOPS	natural abundance	20 mM
6	H2O	natural abundance	95 %
7	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 1.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 1.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 4YYP, Strand ID: A, B Detail

Release date

2015-08-06

Citation

STIL binding to Polo-box 3 of PLK4 regulates centriole duplication
Arquint, C., Gabryjonczyk, A., Imseng, S., Boehm, R., Sauer, E., Hiller, S., Nigg, E., Maier, T.
Elife (2015), 4, PubMed 26188084 , DOI 10.7554/eLife.07888 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25552 released on 2015-08-06
Title STIL binding to Polo-box 3 of PLK4 regulates centriole duplication - NMR solution structure of human Polo-box 3

Related entities 1. PLK4-PB3, : 1 : 3 : 2 : 27 entities Detail

Related entities 2. PLK4-PB3, : 1 : 2 : 4 : 5 entities Detail

Interaction partners 1. PLK4-PB3, : 24 : 1 interactors Detail

More details for 25 interactors identified by 11 citations

Interaction partners 2. PLK4-PB3, : 5 : 1 interactors Detail

More details for 6 interactors identified by 3 citations

Experiments performed 5 experiments Detail

1 2D 1H-15N HSQC