BMRB: 26574

Tom1 negatively modulates binding of Tollip to phosphatidylinositol 3-phosphate via a coupled folding and binding mechanism

Authors

Xiao, S., Capelluto, D.

Assembly

GAT

Entity

1. GAT (polymer, Thiol state: unknown), 100 monomers, 11489.99 Da Detail

GPLGSEQIGK LRSELEMVSG NVRVMSEMLT ELVPTQAEPA DLELLQELNR TCRAMQQRVL ELIPQIANEQ LTEELLIVND NLNNVFLRHE RFERFRTGQT

Formula weight

11489.99 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 41.3 %, Completeness (bb): 77.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	41.3 % (490 of 1186)	19.9 % (124 of 624)	61.4 % (277 of 451)	80.2 % (89 of 111)
Backbone	77.2 % (457 of 592)	48.3 % (97 of 201)	91.9 % (271 of 295)	92.7 % (89 of 96)
Sidechain	17.4 % (120 of 689)	6.4 % (27 of 423)	37.1 % (93 of 251)	0.0 % (0 of 15)
Aromatic	0.0 % (0 of 34)	0.0 % (0 of 17)	0.0 % (0 of 17)
Methyl	8.6 % (11 of 128)	9.4 % (6 of 64)	7.8 % (5 of 64)

1. GAT

GPLGSEQIGK LRSELEMVSG NVRVMSEMLT ELVPTQAEPA DLELLQELNR TCRAMQQRVL ELIPQIANEQ LTEELLIVND NLNNVFLRHE RFERFRTGQT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	GAT	[U-99% 13C; U-99% 15N]		1.0 mM
2	potassium chloride	natural abundance		50 mM
3	TRIS	[U-2H]		20 mM
4	sodium azide	natural abundance		1 mM
5	DSS	natural abundance		50 uM
6	DTT	[U-2H]		1 mM

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LACS Plot; CA

Referencing offset: 2.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 1.99 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 2N9D, Strand ID: A Detail

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Release date

2015-12-21

Citation

Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism
Xiao, S., Brannon, M.K., Zhao, X., Fread, K.I., Ellena, J.F., Bushweller, J.H., Finkielstein, C.V., Armstrong, G.S., Capelluto, D.G.S.
Structure (2015), 23, 1910-1920, PubMed 26320582 , DOI 10.1016/j.str.2015.07.017 , Abstract

Related entities 1. GAT, : 1 : 2 : 2 : 46 entities Detail

Interaction partners 1. GAT, : 19 interactors Detail

Experiments performed 5 experiments Detail