BMRB: 26649

1H, 13C, 15N chemical shift assignments of initiation factor 1 from Pseudomonas aeruginosa

Authors

Zhang, Y.

Assembly

IF1 monomer

Entity

1. IF1 monomer (polymer, Thiol state: not present), 80 monomers, 9367.564 Da Detail

MSKEDSFEME GTVVDTLPNT MFRVELENGH VVTAHISGKM RKNYIRILTG DKVRVELTPY DLSKGRITYR ARLEHHHHHH

Formula weight

9367.564 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.7 %, Completeness: 77.2 %, Completeness (bb): 88.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	77.2 % (729 of 944)	77.6 % (381 of 491)	74.5 % (277 of 372)	87.7 % (71 of 81)
Backbone	88.2 % (420 of 476)	89.0 % (145 of 163)	87.7 % (206 of 235)	88.5 % (69 of 78)
Sidechain	69.1 % (375 of 543)	72.0 % (236 of 328)	64.6 % (137 of 212)	66.7 % (2 of 3)
Aromatic	21.1 % (16 of 76)	31.6 % (12 of 38)	10.5 % (4 of 38)
Methyl	94.2 % (81 of 86)	95.3 % (41 of 43)	93.0 % (40 of 43)

1. Initiation Factor IF-1

MSKEDSFEME GTVVDTLPNT MFRVELENGH VVTAHISGKM RKNYIRILTG DKVRVELTPY DLSKGRITYR ARLEHHHHHH

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.1

#	Name	Isotope labeling	Type	Concentration
1	Initiation Factor IF-1	[U-100% 13C; U-100% 15N]		1 mM
2	KPO4	natural abundance		25 mM
3	KCl	natural abundance		100 mM

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LACS Plot; CA

Referencing offset: 0.13 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.13 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.54 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 15 models in PDB: 2N78, Strand ID: A Detail

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Release date

2016-08-21

Citation

¹H, ¹³C and ¹⁵N resonance assignments and secondary structure analysis of translation initiation factor 1 from Pseudomonas aeruginosa
Bernal, A., Hu, Y., Palmer, S.O., Silva, A., Bullard, J., Zhang, Y.
Biomol. NMR Assign. (2016), 10, 249-252, PubMed 26983940 , DOI 10.1007/s12104-016-9678-7 , Abstract

Related entities 1. IF1 monomer, : 1 : 2 : 128 entities Detail

Experiments performed 5 experiments Detail