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Structural basis for the inhibition of voltage-gated sodium channels with conotoxin-muOxi-GVIIJ
Authors
Green, B.R., Chhabra, S., Norton, R.S.
Assembly
GVIIJ[C24S]
Entity
1. GVIIJ[C24S] (polymer, Thiol state: all disulfide bound), 35 monomers, 3729.205 Da Detail

GWCGDXGATC GKLRLYCCSG FCDSYTKTCK DKSSA


Formula weight
3729.205 Da
Entity Connection
disulfide 3 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS3:SG1:CYS18:SG
2disulfidesing1:CYS10:SG1:CYS29:SG
3disulfidesing1:CYS17:SG1:CYS29:SG

Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.1 %, Completeness: 75.0 %, Completeness (bb): 78.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All75.0 % (267 of 356)93.4 % (171 of 183)47.1 % (65 of 138)88.6 % (31 of 35)
Backbone78.4 % (160 of 204)98.6 % (72 of 73)58.8 % (57 of 97)91.2 % (31 of 34)
Sidechain72.9 % (132 of 181)90.0 % (99 of 110)47.1 % (33 of 70) 0.0 % (0 of 1)
Aromatic68.4 % (26 of 38)100.0 % (19 of 19)38.9 % (7 of 18) 0.0 % (0 of 1)
Methyl55.6 % (10 of 18)77.8 % (7 of 9)33.3 % (3 of 9)

1. GVIIJ C24S

GWCGDXGATC GKLRLYCCSG FCDSYTKTCK DKSSA

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 3.2


#NameIsotope labelingTypeConcentration
1GVIIJ_C24Snatural abundance1.4 mM
2H2Onatural abundance90 %
3D2Onatural abundance10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2N8H, Strand ID: A Detail


Release date
2016-01-31
Citation
Structural Basis for the Inhibition of Voltage-gated Sodium Channels by Conotoxin μO§-GVIIJ
Green, B.R., Gajewiak, J.R., Chhabra, S., Skalicky, J.J., Zhang, M., Bulaj, G., Olivera, B.M., Yoshikami, D., Norton, R.S.
J. Biol. Chem. (2016), 291, 7205-7220, PubMed 26817840 , DOI 10.1074/jbc.M115.697672 ,
Related entities 1. GVIIJ[C24S], : 1 : 7 entities Detail
Experiments performed 5 experiments Detail
NMR combined restraints 5 contents Detail