BMRB: 26692

Solid State NMR sequential assignment of Amyloid b(1-42) fibrils

Authors

Ravotti, F., Waelti, M.A., Guentert, P., Meier, B.H., Riek, R., Boeckmann, A.

Assembly

Amyloid Beta fibril

Entity

1. Amyloid Beta fibril (polymer, Thiol state: not present), 42 monomers, 4514.037 Da Detail

DAEFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVV IA

Formula weight

4514.037 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.5 %, Completeness: 84.8 %, Completeness (bb): 90.1 % Detail

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Polymer type: polypeptide(L)

	Total	13C	15N
All	84.8 % (195 of 230)	83.3 % (155 of 186)	90.9 % (40 of 44)
Backbone	90.1 % (146 of 162)	90.0 % (108 of 120)	90.5 % (38 of 42)
Sidechain	77.9 % (81 of 104)	77.5 % (79 of 102)	100.0 % (2 of 2)
Aromatic	48.0 % (12 of 25)	48.0 % (12 of 25)
Methyl	92.3 % (24 of 26)	92.3 % (24 of 26)

1. Amyloid Beta

DAEFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVV IA

Show sample condition

Sample

Solvent system Sodium phosphate, Pressure 1 atm, Temperature 273 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	Amyloid Beta	[U-13C]		20 mg

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Calculated from 10 models in PDB: 2NAO, Strand ID: A, B, C, D, E, F Detail

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Release date

2016-05-23

Citation

Solid-state NMR sequential assignment of an Amyloid-β(1-42) fibril polymorph
Ravotti, F., Waelti, M.A., Guentert, P., Meier, B.H., Riek, R., Boeckmann, A.
Biomol. NMR Assign. (2016), 10, 269-276, PubMed 27165577 , DOI 10.1007/s12104-016-9682-y , Abstract

Related entities 1. Amyloid Beta fibril, : 1 : 51 : 3 : 59 : 17 entities Detail

Interaction partners 1. Amyloid Beta fibril, : 96 interactors Detail

Experiments performed 8 experiments Detail