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Found 1 Document (1.299 seconds)

BMRB: 27095

6BUT

Backbone and side chain chemical shift assignments of apo calmodulin bound to the NaV1.2 IQ motif peptide

Authors

Mahling, R., Kilpatrick, A.M., Shea, M.A.

Assembly

CaM NaV1.2 IQ motif

Entity

1. Calmodulin (polymer, Thiol state: not present), 148 monomers, 16706.21 × 2 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. Voltage-gated sodium channel NaV1-2 IQ motif peptide (polymer, Thiol state: not present), 31 monomers, 3672.329 × 2 Da Detail

GPGSKRKQEE VSAIIIQRAY RRYLLKQKVK K

Total weight

40757.08 Da

Max. entity weight

16706.21 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 83.2 %, Completeness: 75.4 %, Completeness (bb): 81.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.4 % (1563 of 2074)	71.6 % (778 of 1087)	79.5 % (633 of 796)	79.6 % (152 of 191)
Backbone	81.4 % (869 of 1068)	80.7 % (297 of 368)	81.9 % (429 of 524)	81.3 % (143 of 176)
Sidechain	70.9 % (831 of 1172)	66.9 % (481 of 719)	77.9 % (341 of 438)	60.0 % (9 of 15)
Aromatic	81.9 % (95 of 116)	84.5 % (49 of 58)	79.3 % (46 of 58)
Methyl	80.5 % (140 of 174)	81.6 % (71 of 87)	79.3 % (69 of 87)

1. Calmodulin

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. Voltage-gated sodium channel NaV1-2 IQ motif peptide

GPGSKRKQEE VSAIIIQRAY RRYLLKQKVK K

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.95 mM
2	Voltage-gated sodium channel NaV1.2 IQ motif peptide	[U-99% 13C; U-99% 15N]	0.95 mM
3	imidazole	[U-99% 2H]	10 mM
4	potassium chloride	natural abundance	100 mM
5	EDTA	natural abundance	0.1 mM
6	sodium azide	natural abundance	0.01 %

Chem. Shift Complete2

Sequence coverage: 35.2 %, Completeness: 52.6 %, Completeness (bb): 57.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.6 % (2180 of 4148)	50.1 % (1089 of 2174)	55.1 % (877 of 1592)	56.0 % (214 of 382)
Backbone	57.3 % (1224 of 2136)	56.8 % (418 of 736)	57.5 % (603 of 1048)	57.7 % (203 of 352)
Sidechain	48.9 % (1147 of 2344)	46.7 % (671 of 1438)	53.1 % (465 of 876)	36.7 % (11 of 30)
Aromatic	48.3 % (112 of 232)	50.0 % (58 of 116)	46.6 % (54 of 116)
Methyl	52.0 % (181 of 348)	52.9 % (92 of 174)	51.1 % (89 of 174)

1. Calmodulin

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. Voltage-gated sodium channel NaV1-2 IQ motif peptide

GPGSKRKQEE VSAIIIQRAY RRYLLKQKVK K

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.95 mM
2	Voltage-gated sodium channel NaV1.2 IQ motif peptide	[U-99% 13C; U-99% 15N]	0.95 mM
3	imidazole	[U-99% 2H]	10 mM
4	potassium chloride	natural abundance	100 mM
5	EDTA	natural abundance	0.1 mM
6	sodium azide	natural abundance	0.01 %

Chem. Shift Complete3

Sequence coverage: 30.7 %, Completeness: 42.1 %, Completeness (bb): 46.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.1 % (2618 of 6222)	40.6 % (1323 of 3261)	43.5 % (1038 of 2388)	44.9 % (257 of 573)
Backbone	46.3 % (1483 of 3204)	46.1 % (509 of 1104)	46.3 % (728 of 1572)	46.6 % (246 of 528)
Sidechain	38.9 % (1367 of 3516)	37.7 % (814 of 2157)	41.2 % (542 of 1314)	24.4 % (11 of 45)
Aromatic	36.5 % (127 of 348)	40.2 % (70 of 174)	32.8 % (57 of 174)
Methyl	41.8 % (218 of 522)	42.9 % (112 of 261)	40.6 % (106 of 261)

1. Calmodulin

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. Voltage-gated sodium channel NaV1-2 IQ motif peptide

GPGSKRKQEE VSAIIIQRAY RRYLLKQKVK K

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.95 mM
2	Voltage-gated sodium channel NaV1.2 IQ motif peptide	[U-99% 13C; U-99% 15N]	0.95 mM
3	imidazole	[U-99% 2H]	10 mM
4	potassium chloride	natural abundance	100 mM
5	EDTA	natural abundance	0.1 mM
6	sodium azide	natural abundance	0.01 %

Chem. Shift Complete4

Sequence coverage: 27.4 %, Completeness: 36.3 %, Completeness (bb): 40.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	36.3 % (3009 of 8296)	35.4 % (1538 of 4348)	36.7 % (1170 of 3184)	39.4 % (301 of 764)
Backbone	40.2 % (1718 of 4272)	40.9 % (602 of 1472)	39.5 % (827 of 2096)	41.1 % (289 of 704)
Sidechain	33.3 % (1559 of 4688)	32.5 % (936 of 2876)	34.9 % (611 of 1752)	20.0 % (12 of 60)
Aromatic	31.9 % (148 of 464)	35.8 % (83 of 232)	28.0 % (65 of 232)
Methyl	35.8 % (249 of 696)	37.1 % (129 of 348)	34.5 % (120 of 348)

1. Calmodulin

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. Voltage-gated sodium channel NaV1-2 IQ motif peptide

GPGSKRKQEE VSAIIIQRAY RRYLLKQKVK K

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.95 mM
2	Voltage-gated sodium channel NaV1.2 IQ motif peptide	[U-99% 13C; U-99% 15N]	0.95 mM
3	imidazole	[U-99% 2H]	10 mM
4	potassium chloride	natural abundance	100 mM
5	EDTA	natural abundance	0.1 mM
6	sodium azide	natural abundance	0.01 %

Protein Blocks Logo

Calculated from 20 models in PDB: 6BUT, Strand ID: A, B Detail

Release date

2017-05-07

Citation

Backbone resonance assignments of complexes of human voltage-dependent sodium channel Na_V1.2 IQ motif peptide bound to apo calmodulin and to the C-domain fragment of apo calmodulin
Mahling, R., Kilpatrick, A.M., Shea, M.A.
Biomol. NMR Assign. (2017), 11, 297-303, PubMed 28823028 , DOI 10.1007/s12104-017-9767-2 , Abstract

Related entities 1. Calmodulin, : 11 : 88 entities Detail

Related entities 2. Voltage-gated sodium channel NaV1-2 IQ motif peptide, : 1 : 3 : 71 entities Detail

Experiments performed 4 experiments Detail

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Found 1 Document (1.299 seconds)

BMRB: 27095

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Sample

Sample

Related entities 1. Calmodulin, : 11 : 88 entities Detail

Related entities 2. Voltage-gated sodium channel NaV1-2 IQ motif peptide, : 1 : 3 : 71 entities Detail

Experiments performed 4 experiments Detail

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