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BMRB: 27146

6Q44

Est3 Hansenula polymorpha telomerase subunit

Authors

Polshakov, V., Mariasina, S., Efimov, S., Petrova, O.

Assembly

hpEst3

Entity

1. hpEst3 (polymer, Thiol state: all free), 178 monomers, 20454.25 Da Detail

GAMGPPSSRD AVRVTASAHM KHWLEPVLCE AGLGHNYKVD KVLKVLRIYP RSNTLSSLPL CLCDANYKIL AFANYKAIAA FERKERRRVT QNLLNSEIMI HSFTIRFYND DQVQGFFDGL KFKQKASLFP GYLVLEINDF SMFNRDQLIL SNAGTIEFLY GTPRYIARFI EQEFSDEE

Formula weight

20454.25 Da

Source organism

Ogataea angusta

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.6 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.6 % (1839 of 2148)	86.9 % (971 of 1118)	81.7 % (688 of 842)	95.7 % (180 of 188)
Backbone	97.0 % (1022 of 1054)	96.4 % (345 of 358)	97.3 % (511 of 525)	97.1 % (166 of 171)
Sidechain	77.2 % (975 of 1263)	81.8 % (622 of 760)	70.0 % (340 of 486)	76.5 % (13 of 17)
Aromatic	50.0 % (111 of 222)	84.7 % (94 of 111)	14.5 % (16 of 110)	100.0 % (1 of 1)
Methyl	88.3 % (173 of 196)	88.8 % (87 of 98)	87.8 % (86 of 98)

1. Est3

GAMGPPSSRD AVRVTASAHM KHWLEPVLCE AGLGHNYKVD KVLKVLRIYP RSNTLSSLPL CLCDANYKIL AFANYKAIAA FERKERRRVT QNLLNSEIMI HSFTIRFYND DQVQGFFDGL KFKQKASLFP GYLVLEINDF SMFNRDQLIL SNAGTIEFLY GTPRYIARFI EQEFSDEE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details H2O

#	Name	Isotope labeling	Concentration
1	Est3	[U-99% 13C; U-99% 15N]	0.5 mM
2	potassium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	50 mM
4	DTT	natural abundance	3 mM
5	sodium azide	natural abundance	0.02 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details D2O

#	Name	Isotope labeling	Concentration
6	Est3	[U-99% 13C; U-99% 15N]	0.7 mM
7	potassium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	50 mM
9	DTT	natural abundance	3 mM
10	sodium azide	natural abundance	0.02 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details H2O

#	Name	Isotope labeling	Concentration
11	Est3	[U-99% 15N]	0.8 mM
12	potassium chloride	natural abundance	100 mM
13	sodium phosphate	natural abundance	50 mM
14	DTT	natural abundance	3 mM
15	sodium azide	natural abundance	0.02 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details D2O

#	Name	Isotope labeling	Concentration
16	Est3	natural abundance	1.0 mM
17	potassium chloride	natural abundance	100 mM
18	sodium phosphate	natural abundance	50 mM
19	DTT	natural abundance	3 mM
20	sodium azide	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.33 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6Q44, Strand ID: A Detail

Release date

2017-06-18

Citation

Chemical shift assignments and the secondary structure of the Est3 telomerase subunit in the yeast Hansenula polymorpha
Mariasina, S.S., Efimov, S.V., Petrova, O.A., Rodina, E.V., Malyavko, A.N., Zvereva, M.I., Klochkov, V.V., Dontsova, O.A., Polshakov, V.I.
Biomol. NMR Assign. (2017), 12, 57-62, PubMed 28916982 , DOI 10.1007/s12104-017-9780-5 , Abstract