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BMRB: 27271

6B7G

Solution NMR structure of BCoR in complex with AF9 (BCoR-AF9)

Authors

Schmidt, C.R., Kuntimaddi, A., Leach, B.I., Bushweller, J.H.

Assembly

BCoR-AF9

Entity

1. AF9 (polymer, Thiol state: all free), 70 monomers, 8266.313 Da Detail

MDKAYLDELV ELHRRLMTLR ERHILQQIVN LIEETGHFHI TNTTFDFDLC SLDKTTVRKL QSYLETSGTS

2. BCoR (polymer, Thiol state: all free), 34 monomers, 3764.136 Da Detail

GTTNSSSNHL EDPHYSELTN LKVCIELTGL HPKK

Total weight

12030.449 Da

Max. entity weight

8266.313 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 66.3 %, Completeness: 64.4 %, Completeness (bb): 66.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	64.4 % (788 of 1223)	63.9 % (404 of 632)	64.7 % (311 of 481)	66.4 % (73 of 110)
Backbone	66.1 % (410 of 620)	65.7 % (138 of 210)	66.2 % (204 of 308)	66.7 % (68 of 102)
Sidechain	63.3 % (445 of 703)	63.0 % (266 of 422)	63.7 % (174 of 273)	62.5 % (5 of 8)
Aromatic	53.7 % (44 of 82)	53.7 % (22 of 41)	53.7 % (22 of 41)
Methyl	71.9 % (92 of 128)	71.9 % (46 of 64)	71.9 % (46 of 64)

1. AF9

MDKAYLDELV ELHRRLMTLR ERHILQQIVN LIEETGHFHI TNTTFDFDLC SLDKTTVRKL QSYLETSGTS

2. BCoR

GTTNSSSNHL EDPHYSELTN LKVCIELTGL HPKK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	AF9	[U-100% 13C; U-100% 15N]	750 uM
2	BCoR	[U-100% 13C; U-100% 15N]	750 uM
3	Bis-Tris	natural abundance	9.3 mM
4	MES	natural abundance	15.8 mM
5	DTT	natural abundance	1 mM
6	D2O	[U-2H]	5 %
7	H2O	natural abundance	95 %
8	NaCl	natural abundance	100 mM

Chem. Shift Complete2

Sequence coverage: 43.3 %, Completeness: 49.5 %, Completeness (bb): 52.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	49.5 % (1211 of 2446)	48.1 % (608 of 1264)	50.6 % (487 of 962)	52.7 % (116 of 220)
Backbone	52.9 % (656 of 1240)	51.9 % (218 of 420)	53.2 % (328 of 616)	53.9 % (110 of 204)
Sidechain	47.2 % (664 of 1406)	46.2 % (390 of 844)	49.1 % (268 of 546)	37.5 % (6 of 16)
Aromatic	31.1 % (51 of 164)	29.3 % (24 of 82)	32.9 % (27 of 82)
Methyl	53.1 % (136 of 256)	52.3 % (67 of 128)	53.9 % (69 of 128)

1. AF9

MDKAYLDELV ELHRRLMTLR ERHILQQIVN LIEETGHFHI TNTTFDFDLC SLDKTTVRKL QSYLETSGTS

2. BCoR

GTTNSSSNHL EDPHYSELTN LKVCIELTGL HPKK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	AF9	[U-100% 13C; U-100% 15N]	750 uM
2	BCoR	[U-100% 13C; U-100% 15N]	750 uM
3	Bis-Tris	natural abundance	9.3 mM
4	MES	natural abundance	15.8 mM
5	DTT	natural abundance	1 mM
6	D2O	[U-2H]	5 %
7	H2O	natural abundance	95 %
8	NaCl	natural abundance	100 mM

Release date

2017-10-03

Citation

Direct Recruitment of BCoR, but not CBX8, is Required for MLL-AF9 Leukemia
Schmidt, C.R., Achille, N.J., Kuntimaddi, A., Boulton, A.M., Leach, B.I., Zeleznik-Le, N.J., Bushweller, J.H.
Not known

Related entities 1. AF9, : 5 : 19 entities Detail

Related entities 2. BCoR, : 1 : 2 : 5 entities Detail

Interaction partners 1. AF9, : 3 : 1 interactors Detail

More details for 4 interactors identified by 1 citations

Interaction partners 2. BCoR, : 34 : 1 interactors Detail

More details for 35 interactors identified by 9 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC