BMRBj - BMREntryMaster

Found 1 Document (0.467 seconds)

BMRB: 27396

6CCJ

NMR assignments of Rous sarcoma virus matrix protein (M domain)

Authors

Vlach, J., Saad, J., Eastep, G., Ghanam, R.

Assembly

sMA

Entity

1. sMA (polymer, Thiol state: all free), 87 monomers, 9198.681 Da Detail

SEAVIKVISS ACKTYCGKTS PSKKEIGAML SLLQKEGLLM SPSDLYSPGS WDPITAALSQ RAMILGKSGE LKTWGLVLGA LKAAREE

Formula weight

9198.681 Da

Source organism

Rous sarcoma virus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.3 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.3 % (965 of 982)	97.8 % (499 of 510)	98.4 % (379 of 385)	100.0 % (87 of 87)
Backbone	98.6 % (507 of 514)	98.3 % (175 of 178)	98.4 % (249 of 253)	100.0 % (83 of 83)
Sidechain	98.0 % (536 of 547)	97.6 % (324 of 332)	98.6 % (208 of 211)	100.0 % (4 of 4)
Aromatic	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (18 of 18)	100.0 % (2 of 2)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. sRSV MA

SEAVIKVISS ACKTYCGKTS PSKKEIGAML SLLQKEGLLM SPSDLYSPGS WDPITAALSQ RAMILGKSGE LKTWGLVLGA LKAAREE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 305 K, pH 6.000

#	Name	Isotope labeling	Concentration
1	sRSV MA	[U-95% 13C; U-90% 15N]	.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	TCEP	natural abundance	2 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 305 K, pH 6.000

#	Name	Isotope labeling	Concentration
5	sRSV MA	[U-95% 13C; U-90% 15N]	.5 mM
6	sodium phosphate	natural abundance	50 mM
7	sodium chloride	natural abundance	50 mM
8	TCEP	natural abundance	2 mM

Sample #3

Solvent system 100% D2O, Pressure 1.000 atm, Temperature 305 K, pH 6.000

#	Name	Isotope labeling	Concentration
9	sRSV MA	[U-95% 13C; U-90% 15N]	.5 mM
10	sodium phosphate	natural abundance	50 mM
11	potassium chloride	natural abundance	50 mM
12	TCEP	natural abundance	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.56 ppm, Outliers: 1 Detail

Release date

2018-02-06

Citation

Structural basis for targeting avian sarcoma virus Gag polyprotein to the plasma membrane for virus assembly
Vlach, J., Eastep, G.N., Ghanam, R.H., Watanabe, S.M., Carter, C.A., Saad, J.S.
J. Biol. Chem. (2018), 293, 18828-18840, PubMed 30309983 , DOI 10.1074/jbc.RA118.003944 , Abstract

Related entities 1. sMA, : 1 : 4 : 6 : 11 entities Detail

Interaction partners 1. sMA, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 3D 1H-15N NOESY