BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	90.1 % (1354 of 1502)	88.9 % (705 of 793)	91.5 % (528 of 577)	91.7 % (121 of 132)
Backbone	94.6 % (679 of 718)	93.8 % (227 of 242)	94.7 % (342 of 361)	95.7 % (110 of 115)
Sidechain	87.0 % (784 of 901)	86.8 % (478 of 551)	88.6 % (295 of 333)	64.7 % (11 of 17)
Aromatic	77.1 % (111 of 144)	77.8 % (56 of 72)	77.5 % (55 of 71)	0.0 % (0 of 1)
Methyl	98.2 % (110 of 112)	98.2 % (55 of 56)	98.2 % (55 of 56)

1. Lpp1663

GSNQQLNATK KDKLQVIDWL IENFPNAFFK KGNQVKPLKI GIFDDLIDFY ERLDTPPFSK KSLREALSYY SASPAYLSCQ KPDTARVDIY GNEVDVVTPE QAKYAYQRYQ ERYGNKKSQD LK

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Lpp1663	[U-15N]	400 uM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	D2O	natural abundance	10 %
5	DSS	natural abundance	50 uM
6	beta-mercaptoethanol	natural abundance	2 mM

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Lpp1663	[U-13C; U-15N]	400 uM
8	sodium phosphate	natural abundance	50 mM
9	sodium chloride	natural abundance	100 mM
10	D2O	natural abundance	10 %
11	DSS	natural abundance	50 uM
12	beta-mercaptoethanol	natural abundance	2 mM

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Lpp1663	[U-15N]-Lys	400 uM
14	sodium phosphate	natural abundance	50 mM
15	sodium chloride	natural abundance	100 mM
16	D2O	natural abundance	10 %
17	DSS	natural abundance	50 uM
18	beta-mercaptoethanol	natural abundance	2 mM

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
19	Lpp1663	[U-15N]-Tyr	400 uM
20	sodium phosphate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	D2O	natural abundance	10 %
23	DSS	natural abundance	50 uM
24	beta-mercaptoethanol	natural abundance	2 mM

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
25	Lpp1663	[U-15N]-Phe [U-13C]-Pro	400 uM
26	sodium phosphate	natural abundance	50 mM
27	sodium chloride	natural abundance	100 mM
28	D2O	natural abundance	10 %
29	DSS	natural abundance	50 uM
30	beta-mercaptoethanol	natural abundance	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6S10, Strand ID: A Detail

Release date

2018-04-15

Citation

NMR resonance assignments for a ProQ homolog from Legionella pneumophila
Immer, C., Hacker, C., Woehnert, J.
Biomol. NMR Assign. (2018), 12, 319-322, PubMed 29934867 , DOI 10.1007/s12104-018-9831-6 , Abstract

1 2D 1H-15N HSQC