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BMRB: 27503

6RJI

Backbone and side chain NMR assignments for the ribosome Elongation Factor P (EfP) from Staphylococcus aureus

Authors

Golubev, A., Validov, S., Klochkov, V., Aganov, A., Khusainov, I., Yusupov, M.

Assembly

Ef-P

Entity

1. Ef-P (polymer, Thiol state: not present), 185 monomers, 20553.81 Da Detail

MISVNDFKTG LTISVDNAIW KVIDFQHVKP GKGSAFVRSK LRNLRTGAIQ EKTFRAGEKV EPAMIENRRM QYLYADGDNH VFMDNESFEQ TELSSDYLKE ELNYLKEGME VQIQTYEGET IGVELPKTVE LTVTETEPGI KGDTATGATK SATVETGYTL NVPLFVNEGD VLIINTGDGS YISRG

Formula weight

20553.81 Da

Source organism

Staphylococcus aureus

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.8 %, Completeness: 65.3 %, Completeness (bb): 88.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	65.3 % (1384 of 2121)	58.9 % (645 of 1096)	69.8 % (578 of 828)	81.7 % (161 of 197)
Backbone	88.2 % (970 of 1100)	86.9 % (332 of 382)	89.2 % (480 of 538)	87.8 % (158 of 180)
Sidechain	47.4 % (564 of 1189)	43.8 % (313 of 714)	54.1 % (248 of 458)	17.6 % (3 of 17)
Aromatic	26.0 % (38 of 146)	38.4 % (28 of 73)	12.5 % (9 of 72)	100.0 % (1 of 1)
Methyl	56.1 % (120 of 214)	56.1 % (60 of 107)	56.1 % (60 of 107)

1. Ef-P

MISVNDFKTG LTISVDNAIW KVIDFQHVKP GKGSAFVRSK LRNLRTGAIQ EKTFRAGEKV EPAMIENRRM QYLYADGDNH VFMDNESFEQ TELSSDYLKE ELNYLKEGME VQIQTYEGET IGVELPKTVE LTVTETEPGI KGDTATGATK SATVETGYTL NVPLFVNEGD VLIINTGDGS YISRG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

LACS Plot; CA

Referencing offset: 2.37 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 2.37 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.59 ppm, Outliers: 1 Detail

Release date

2018-06-03

Citation

Backbone and side chain NMR assignments for the ribosome Elongation Factor P (EF-P) from Staphylococcus aureus
Usachev, K., Golubev, A., Validov, S., Klochkov, V., Aganov, A., Khusainov, I., Yusupov, M.
Biomol. NMR Assign. (2018), 12, 351-355, PubMed 30099718 , DOI 10.1007/s12104-018-9838-z , Abstract

Related entities 1. Ef-P, : 1 : 3 : 125 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

3 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

4 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

5 2D 1H-1H NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

7 3D C(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

8 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

9 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

10 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

11 3D HBHA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

12 3D H(CCO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

13 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

14 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

15 3D 1H-15N TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 308 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	Ef-P	[U-100% 13C; U-100% 15N]		1.3 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr27503_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr27503_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list	Warning	1382		88.1 (chain: 1, length: 185)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 185, Sequence coverage: 88.1%
- Total number of assignments
  - ¹H chemical shifts: 674
  - ¹³C chemical shifts: 556
  - ¹⁵N chemical shifts: 152
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords EfP, Elongation Factor P, Staphylococcus aureus, ribosome