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BMRB: 27532

6YE5

Backbone and side chain NMR assignments for the ribosome Binding Factor A (RbfA) from Staphylococcus aureus

Authors

Usachev, K.S., Blokhin, D.S., Bikmullin, A.G., Nurullina, L.I., Validov, S.Z., Garaeva, N.S., Klochkov, V.V., Aganov, A.V., Khusainov, I.S.H., Yusupov, M.M.

Assembly

RbfA

Entity

1. RbfA (polymer, Thiol state: not present), 118 monomers, 13668.65 Da Detail

GSSMRAERVG EQMKKELMDI INNKVKDPRV GFITITDVVL TNDLSQAKVF LTVLGNDKEV ENTFKALDKA KGFIKSELGS RMRLRIMPEL MYEYDQSIEY GNKIERMIQD LHKQDRVE

Formula weight

13668.65 Da

Source organism

Staphylococcus aureus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 88.1 %, Completeness: 58.2 %, Completeness (bb): 82.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	58.2 % (831 of 1428)	50.4 % (380 of 754)	63.8 % (349 of 547)	80.3 % (102 of 127)
Backbone	82.8 % (583 of 704)	79.3 % (191 of 241)	85.6 % (297 of 347)	81.9 % (95 of 116)
Sidechain	40.5 % (338 of 835)	36.6 % (188 of 513)	46.0 % (143 of 311)	63.6 % (7 of 11)
Aromatic	20.6 % (14 of 68)	41.2 % (14 of 34)	0.0 % (0 of 34)
Methyl	39.2 % (51 of 130)	38.5 % (25 of 65)	40.0 % (26 of 65)

1. RbfA

GSSMRAERVG EQMKKELMDI INNKVKDPRV GFITITDVVL TNDLSQAKVF LTVLGNDKEV ENTFKALDKA KGFIKSELGS RMRLRIMPEL MYEYDQSIEY GNKIERMIQD LHKQDRVE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 308.000 K, pH 7.600

#	Name	Isotope labeling	Concentration
1	RbfA	[U-100% 13C; U-100% 15N]	0.9 mM
2	phosphate buffer	natural abundance	50 mM
3	NH4Cl	natural abundance	250 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: -0.15 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: -0.15 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Coupling constant

72 J values in 1 lists
Field strength (¹H) 700 MHz, Pressure 1.000 atm, Temperature 308.000 K, pH 7.600 Detail

Release date

2018-07-02

Citation

Backbone and side chain NMR assignments for the ribosome binding factor A (RbfA) from Staphylococcus aureus
Blokhin, D.S., Bikmullin, A., Nurullina, L., Garaeva, N., Validov, S., Klochkov, V., Aganov, A., Khusainov, I., Yusupov, M., Usachev, K.
Biomol. NMR Assign. (2018), 13, 27-30, PubMed 30225569 , DOI 10.1007/s12104-018-9845-0 , Abstract

Related entities 1. RbfA, : 1 : 4 : 112 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC/HMQC