BMRB: 27540

1H, 15N, and 13C triple resonance assignments of innate immune evasion protein EapH2 from the S. aureus

Authors

Herrera, A.I., Dubey, A., Geisbrecht, B.V., Arthanari, H., Prakash, O.

Assembly

Extracellular Adherence Protein Homolog 2

Entity

1. Extracellular Adherence Protein Homolog 2 (polymer, Thiol state: not present), 117 monomers, 13104.75 Da Detail

GSTAEKDKLP ATQKAKEMQN VPYTIAVDGI MAFNQSYLNL PKDSQLSYLD LGNKVKALLY DERGVTPEKI RNAKSAVYTI TWKDGSKKEV DLKKDSYTAN LFDSNSIKQI DINVKTK

Formula weight

13104.75 Da

Source organism

Staphylococcus aureus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 45.3 %, Completeness (bb): 79.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	45.3 % (629 of 1390)	23.6 % (172 of 728)	64.5 % (345 of 535)	88.2 % (112 of 127)
Backbone	79.8 % (554 of 694)	51.1 % (120 of 235)	93.1 % (322 of 346)	99.1 % (112 of 113)
Sidechain	22.5 % (182 of 808)	10.5 % (52 of 493)	43.2 % (130 of 301)	0.0 % (0 of 14)
Aromatic	10.0 % (8 of 80)	12.5 % (5 of 40)	7.7 % (3 of 39)	0.0 % (0 of 1)
Methyl	16.2 % (21 of 130)	10.8 % (7 of 65)	21.5 % (14 of 65)

1. Extracellular Adherence Protein Homolog 2

GSTAEKDKLP ATQKAKEMQN VPYTIAVDGI MAFNQSYLNL PKDSQLSYLD LGNKVKALLY DERGVTPEKI RNAKSAVYTI TWKDGSKKEV DLKKDSYTAN LFDSNSIKQI DINVKTK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Extracellular Adherence Protein Homolog 2	[U-99% 13C; U-99% 15N]		0.75 mM
2	sodium phosphate	natural abundance		50 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	Extracellular Adherence Protein Homolog 2	[U-99% 15N]		0.75 mM
4	sodium phosphate	natural abundance		50 mM

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LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.74 ppm	internal	indirect	0.2514
1H	water	protons	4.74 ppm	internal	direct	1.0
15N	water	protons	4.74 ppm	internal	indirect	0.1013

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LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.74 ppm	internal	indirect	0.2514
1H	water	protons	4.74 ppm	internal	direct	1.0
15N	water	protons	4.74 ppm	internal	indirect	0.1013

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LACS Plot; CO

Referencing offset: 0.41 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.74 ppm	internal	indirect	0.2514
1H	water	protons	4.74 ppm	internal	direct	1.0
15N	water	protons	4.74 ppm	internal	indirect	0.1013

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Release date

2018-07-11

Citation

Backbone resonance assignments of innate immune evasion protein EapH2 from the S. aureus
Herrera, A.I., Dubey, A., Geisbrecht, B.V., Arthanari, H., Prakash, O.
Biomol. NMR Assign. (2019), 13, 219-222, PubMed 30729401 , DOI 10.1007/s12104-019-09880-3 , Abstract

Related entities 1. Extracellular Adherence Protein Homolog 2, : 1 : 3 : 2 : 48 entities Detail

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail