Backbone chemical shift assignments of H2A in H2A-H2B dimer
SGRGKGGKVK GKAKSRSNRA GLQFPVGRIH RLLRKGNYAE RVGAGAPVYL AAVMEYLAAE VLELAGNAAR DNKKTRIIPR HLQLAIRNDE ELNKLLSGVT IAQGGVLPNI QAVLLPKKTE KKA
IPPKTSGKAA KKAGKAQKNI TKTDKKKKRK RKESYAIYIY KVLKQVHPDT GISSKAMSIM NSFVNDIFER IAAEASRLAH YNKRSTITSR EIQTAVRLLL PGELAKHAVS EGTKAVTKYT SSK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 22.3 % (645 of 2891) | 11.2 % (171 of 1524) | 32.0 % (356 of 1112) | 46.3 % (118 of 255) |
Backbone | 40.5 % (591 of 1458) | 25.9 % (130 of 502) | 48.0 % (345 of 719) | 48.9 % (116 of 237) |
Sidechain | 9.8 % (163 of 1660) | 4.1 % (42 of 1022) | 19.2 % (119 of 620) | 11.1 % (2 of 18) |
Aromatic | 0.0 % (0 of 114) | 0.0 % (0 of 57) | 0.0 % (0 of 57) | |
Methyl | 10.5 % (31 of 294) | 6.1 % (9 of 147) | 15.0 % (22 of 147) |
1. H2A
SGRGKGGKVK GKAKSRSNRA GLQFPVGRIH RLLRKGNYAE RVGAGAPVYL AAVMEYLAAE VLELAGNAAR DNKKTRIIPR HLQLAIRNDE ELNKLLSGVT IAQGGVLPNI QAVLLPKKTE KKA2. H2B
IPPKTSGKAA KKAGKAQKNI TKTDKKKKRK RKESYAIYIY KVLKQVHPDT GISSKAMSIM NSFVNDIFER IAAEASRLAH YNKRSTITSR EIQTAVRLLL PGELAKHAVS EGTKAVTKYT SSKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 5.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | H2A | [U-100% 13C; U-100% 15N; U-80% 2H] | 0.3 mM | |
2 | H2B | natural abundance | 0.3 mM | |
3 | MES | natural abundance | 20 mM | |
4 | sodium chloride | natural abundance | 90 mM |