Putative methyltransferase WBSCR27 in apo-form.
GAMAQEEAGR LPQVLARVGT SHGITDLACK LRFYDDWAPE YDQDVAALKY RAPRLAVDCL SRAFRGSPHD ALILDVACGT GLVAVELQAR GFLQVQGVDG SPEMLKQARA RGLYHHLSLC TLGQEPLPDP EGTFDAVIIV GALSEGQVPC SAIPELLRVT KPGGLVCLTT RTNPSNLPYK ETLEATLDSL ERAGVWECLV TQPVDHWELA TSEQETGLGT CANDGFISGI IYLYRKQETV
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 54.1 % (1443 of 2668) | 42.7 % (586 of 1372) | 62.3 % (655 of 1052) | 82.8 % (202 of 244) |
Backbone | 80.9 % (1143 of 1412) | 68.2 % (333 of 488) | 87.5 % (611 of 698) | 88.1 % (199 of 226) |
Sidechain | 32.8 % (483 of 1474) | 28.6 % (253 of 884) | 39.7 % (227 of 572) | 16.7 % (3 of 18) |
Aromatic | 0.0 % (0 of 162) | 0.0 % (0 of 81) | 0.0 % (0 of 78) | 0.0 % (0 of 3) |
Methyl | 37.7 % (116 of 308) | 42.2 % (65 of 154) | 33.1 % (51 of 154) |
1. WBSCR27
GAMAQEEAGR LPQVLARVGT SHGITDLACK LRFYDDWAPE YDQDVAALKY RAPRLAVDCL SRAFRGSPHD ALILDVACGT GLVAVELQAR GFLQVQGVDG SPEMLKQARA RGLYHHLSLC TLGQEPLPDP EGTFDAVIIV GALSEGQVPC SAIPELLRVT KPGGLVCLTT RTNPSNLPYK ETLEATLDSL ERAGVWECLV TQPVDHWELA TSEQETGLGT CANDGFISGI IYLYRKQETVSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | WBSCR27 putative methyltransferase | [U-99% 13C; U-99% 15N] | 0.4 mM | |
2 | NaCl | natural abundance | 50 mM | |
3 | sodium phosphate buffer (pH 7) | natural abundance | 50 mM | |
4 | DTT | natural abundance | 10 mM | |
5 | Sodium azide (NaN3) | natural abundance | 0.02 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | WBSCR27 putative methyltransferase | [U-99% 15N] | 0.8 mM | |
7 | NaCl | natural abundance | 50 mM | |
8 | sodium phosphate buffer (pH 7) | natural abundance | 50 mM | |
9 | DTT | natural abundance | 10 mM | |
10 | Sodium azide (NaN3) | natural abundance | 0.02 % |