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Putative methyltransferase WBSCR27 in apo-form.
Authors
Mariasina, S.S., Polshakov, V.I., Chang, C.
Assembly
assembly 1
Entity
1. assembly 1 (polymer, Thiol state: all free), 240 monomers, 25963.23 Da Detail

GAMAQEEAGR LPQVLARVGT SHGITDLACK LRFYDDWAPE YDQDVAALKY RAPRLAVDCL SRAFRGSPHD ALILDVACGT GLVAVELQAR GFLQVQGVDG SPEMLKQARA RGLYHHLSLC TLGQEPLPDP EGTFDAVIIV GALSEGQVPC SAIPELLRVT KPGGLVCLTT RTNPSNLPYK ETLEATLDSL ERAGVWECLV TQPVDHWELA TSEQETGLGT CANDGFISGI IYLYRKQETV


Formula weight
25963.23 Da
Source organism
Mus musculus
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 92.5 %, Completeness: 54.1 %, Completeness (bb): 80.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All54.1 % (1443 of 2668)42.7 % (586 of 1372)62.3 % (655 of 1052)82.8 % (202 of 244)
Backbone80.9 % (1143 of 1412)68.2 % (333 of 488)87.5 % (611 of 698)88.1 % (199 of 226)
Sidechain32.8 % (483 of 1474)28.6 % (253 of 884)39.7 % (227 of 572)16.7 % (3 of 18)
Aromatic 0.0 % (0 of 162) 0.0 % (0 of 81) 0.0 % (0 of 78) 0.0 % (0 of 3)
Methyl37.7 % (116 of 308)42.2 % (65 of 154)33.1 % (51 of 154)

1. WBSCR27

GAMAQEEAGR LPQVLARVGT SHGITDLACK LRFYDDWAPE YDQDVAALKY RAPRLAVDCL SRAFRGSPHD ALILDVACGT GLVAVELQAR GFLQVQGVDG SPEMLKQARA RGLYHHLSLC TLGQEPLPDP EGTFDAVIIV GALSEGQVPC SAIPELLRVT KPGGLVCLTT RTNPSNLPYK ETLEATLDSL ERAGVWECLV TQPVDHWELA TSEQETGLGT CANDGFISGI IYLYRKQETV

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7


#NameIsotope labelingTypeConcentration
1WBSCR27 putative methyltransferase[U-99% 13C; U-99% 15N]0.4 mM
2NaClnatural abundance50 mM
3sodium phosphate buffer (pH 7)natural abundance50 mM
4DTTnatural abundance10 mM
5Sodium azide (NaN3)natural abundance0.02 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7


#NameIsotope labelingTypeConcentration
6WBSCR27 putative methyltransferase[U-99% 15N]0.8 mM
7NaClnatural abundance50 mM
8sodium phosphate buffer (pH 7)natural abundance50 mM
9DTTnatural abundance10 mM
10Sodium azide (NaN3)natural abundance0.02 %

Release date
2018-08-13
Citation
Williams-Beuren syndrome-related methyltransferase WBSCR27: cofactor binding and cleavage
Mariasina, S.S., Chang, C.F., Petrova, O.A., Efimov, S.V., Klochkov, V.V., Kechko, O.I., Mitkevich, V.A., Sergiev, P.V., Dontsova, O.A., Polshakov, V.I.
FEBS J. (2020), 287, 5375-5393, PubMed 32255258 , DOI 10.1111/febs.15320 ,
Related entities 1. assembly 1, : 1 : 11 entities Detail
Experiments performed 11 experiments Detail
nullKeywords 5'-Methylthioadenosine, NMR assignments, S-Adenosyl-L-homocysteine, S-Adenosyl-L-methionine, Williams-Beuren syndrome, methyltransferase, protein secondary structure