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BMRB: 27579

1PIN

Full Length Apo Pin1

Authors

Born, A., Vogeli, B., Bayer, P., Tate, S., Nichols, P., Henen, M., Strotz, D.

Assembly

Pin1

Entity

1. Pin1 (polymer, Thiol state: all free), 163 monomers, 18243.07 Da Detail

MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE

Formula weight

18243.07 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.0 %, Completeness (bb): 82.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.0 % (1667 of 1874)	95.2 % (942 of 990)	78.2 % (557 of 712)	97.7 % (168 of 172)
Backbone	82.4 % (794 of 964)	96.4 % (322 of 334)	67.5 % (320 of 474)	97.4 % (152 of 156)
Sidechain	95.5 % (1010 of 1058)	93.9 % (616 of 656)	97.9 % (378 of 386)	100.0 % (16 of 16)
Aromatic	97.3 % (142 of 146)	97.3 % (71 of 73)	97.1 % (68 of 70)	100.0 % (3 of 3)
Methyl	99.1 % (115 of 116)	98.3 % (57 of 58)	100.0 % (58 of 58)

1. Pin1

MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE

Show sample condition

Sample

Solvent system 97%H2O/3%D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Pin1	[U-99% 13C; U-99% 15N]	2 mM
2	D2O	natural abundance	3 %
3	DTT	natural abundance	5 mM
4	sodium phosphate	natural abundance	20 mM
5	sodium chloride	natural abundance	50 mM
6	sodium azide	natural abundance	0.03 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	na	methyl carbon	na	indirect	1.0
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	na	nitrogen	na	indirect	1.0

Referencing offset: -0.41 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	na	methyl carbon	na	indirect	1.0
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	na	nitrogen	na	indirect	1.0

Referencing offset: -0.41 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	na	methyl carbon	na	indirect	1.0
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	na	nitrogen	na	indirect	1.0

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Release date

2018-08-15

Citation

Backbone and side-chain chemical shift assignments of full-length, apo, human Pin1, a phosphoprotein regulator with interdomain allostery
Born, A., Nichols, P.J., Henen, M.A., Chi, C.N., Strotz, D., Bayer, P., Tate, S., Peng, J.W., Vogeli, B.
Biomol. NMR Assign. (2018), 13, 85-89, PubMed 30353504 , DOI 10.1007/s12104-018-9857-9 , Abstract

Related entities 1. Pin1, : 1 : 7 : 3 : 42 : 23 entities Detail

Interaction partners 1. Pin1, : 141 interactors Detail

More details for 141 interactors identified by 38 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC