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BMRB: 27581

6HKC

1H, 13C, and 15N chemical shift assignments of the Sushi 1 domain of GABAbR1a

Authors

Volkov, A., Rice, H., Van Molle, I., De Strooper, B., de Wit, J.

Assembly

Sushi1-APPP

Entity

1. Sushi1 (polymer, Thiol state: all disulfide bound), 75 monomers, 8507.615 Da Detail

GPTSEGCQII HPPWEGGIRY RGLTRDQVKA INFLPVDYEI EYVCRGEREV VGPKVRKCLA NGSWTDMDTP SRCVR

2. APPP (polymer, Thiol state: not present), 9 monomers, 1036.010 Da Detail

DDSDVWWGG

Total weight

9543.625 Da

Max. entity weight

8507.615 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS58:SG
2	disulfide	sing	1:CYS44:SG	1:CYS73:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.3 %, Completeness: 87.6 %, Completeness (bb): 86.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.6 % (841 of 960)	88.0 % (442 of 502)	87.6 % (326 of 372)	84.9 % (73 of 86)
Backbone	86.8 % (427 of 492)	86.6 % (149 of 172)	87.2 % (211 of 242)	85.9 % (67 of 78)
Sidechain	88.7 % (481 of 542)	88.8 % (293 of 330)	89.2 % (182 of 204)	75.0 % (6 of 8)
Aromatic	65.1 % (56 of 86)	65.1 % (28 of 43)	66.7 % (26 of 39)	50.0 % (2 of 4)
Methyl	94.7 % (72 of 76)	94.7 % (36 of 38)	94.7 % (36 of 38)

1. Sushi1

GPTSEGCQII HPPWEGGIRY RGLTRDQVKA INFLPVDYEI EYVCRGEREV VGPKVRKCLA NGSWTDMDTP SRCVR

2. APPP

DDSDVWWGG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Sushi1	[U-13C; U-15N]	1 mM
2	APP	natural abundance	3 mM
3	potassium phosphate	natural abundance	50 mM
4	sodium chloride	natural abundance	50 mM
5	sodium azide	natural abundance	0.01 %
6	D2O	[U-100% 2H]	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.44 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6HKC, Strand ID: A Detail

Release date

2018-08-21

Citation

Secreted amyloid-β precursor protein functions as a GABA_BR1a ligand to modulate synaptic transmission
Rice, H.C., de Malmazet, D., Schreurs, A., Frere, S., Van Molle, I., Volkov, A.N., Creemers, E., Vertkin, I., Nys, J., Ranaivoson, F.M., Comoletti, D., Savas, J.N., Remaut, H., Balschun, D., Wierda, K.D., Slutsky, I., Farrow, K., De Strooper, B., de Wit, J.
Science (2019), 363, 4827-4827, PubMed 30630900 , DOI 10.1126/science.aao4827 , Abstract

Related entities 1. Sushi1, : 6 : 4 entities Detail

Related entities 2. APPP, : 13 : 1 : 1 entities Detail

Interaction partners 1. Sushi1, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Interaction partners 2. APPP, : 9 interactors Detail

More details for 9 interactors identified by 8 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC