BMRB: 27664

UbcH7-Ub isopeptide conjugate

Authors

Condos, T.EC., Shaw, G.S.

Assembly

UbcH7-Ub

Entity

1. UbcH7 (polymer, Thiol state: all free), 154 monomers, 17854.25 Da Detail

MAASRRLMKE LEEIRKSGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVKLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFSKNA EEFTKKYGEK RPVD

2. Ub (polymer, Thiol state: all free), 76 monomers, 8556.668 Da Detail

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Total weight

26410.918 Da

Max. entity weight

17854.25 Da

Entity Connection

covalent 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	covalent	sing	1:LYS86:NZ	2:GLY76:C

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 61.3 %, Completeness: 17.4 %, Completeness (bb): 41.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	17.4 % (300 of 1729)	10.7 % (160 of 1490)	58.6 % (140 of 239)
Backbone	41.3 % (276 of 669)	29.9 % (136 of 455)	65.4 % (140 of 214)
Sidechain	2.3 % (24 of 1060)	2.3 % (24 of 1035)	0.0 % (0 of 25)
Aromatic	0.0 % (0 of 89)	0.0 % (0 of 87)	0.0 % (0 of 2)
Methyl	5.8 % (7 of 121)	5.8 % (7 of 121)

1. UbcH7

MAASRRLMKE LEEIRKSGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVKLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFSKNA EEFTKKYGEK RPVD

2. Ub

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	UbcH7	[U-99% 2H]		0.11 (±0.1) mM
2	Ub	[U-99% 2H]		0.11 (±0.1) mM
3	DSS	natural abundance		0.01 mM
4	HEPES	natural abundance		25 (±0.1) mM
5	sodium chloride	natural abundance		50 mM
6	TCEP	natural abundance		0.5 mM

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Chem. Shift Complete2

Sequence coverage: 34.8 %, Completeness: 15.1 %, Completeness (bb): 33.3 % Detail

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• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
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Polymer type: polypeptide(L)

	Total	1H	15N
All	15.1 % (521 of 3458)	10.1 % (301 of 2980)	46.0 % (220 of 478)
Backbone	33.3 % (445 of 1338)	24.8 % (226 of 910)	51.2 % (219 of 428)
Sidechain	3.6 % (76 of 2120)	3.6 % (75 of 2070)	2.0 % (1 of 50)
Aromatic	0.0 % (0 of 178)	0.0 % (0 of 174)	0.0 % (0 of 4)
Methyl	4.5 % (11 of 242)	4.5 % (11 of 242)

1. UbcH7

MAASRRLMKE LEEIRKSGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVKLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFSKNA EEFTKKYGEK RPVD

2. Ub

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	UbcH7	[U-99% 2H]		0.11 (±0.1) mM
2	Ub	[U-99% 2H]		0.11 (±0.1) mM
3	DSS	natural abundance		0.01 mM
4	HEPES	natural abundance		25 (±0.1) mM
5	sodium chloride	natural abundance		50 mM
6	TCEP	natural abundance		0.5 mM

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Release date

2018-10-28

Citation

Synergistic recruitment of UbcH7~Ub and phosphorylated Ubl domain triggers parkin activation
Condos, T.EC., Shaw, G.S.
EMBO J. (2018), PubMed 30446597 , DOI 10.15252/embj.2018100014 , Abstract

Related entities 1. UbcH7, : 3 : 189 entities Detail

Related entities 2. Ub, : 121 : 119 : 186 entities Detail

Interaction partners 1. UbcH7, : 45 interactors Detail

Experiments performed 1 experiments Detail