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BMRB: 27666

6I9B

Resonance assignment of human LARP4A La module

Authors

Cruz-Gallardo, I., Martino, L., Trotta, R., De Tito, S., Atkinson, A., Conte, M.R.

Assembly

LARP4A

Entity

1. LARP4A (polymer, Thiol state: all free), 179 monomers, 20764.63 Da Detail

QSNSAVSTED LKECLKKQLE FCFSRENLSK DLYLISQMDS DQFIPIWTVA NMEEIKKLTT DPDLILEVLR SSPMVQVDEK GEKVRPSHKR CIVILREIPE TTPIEEVKGL FKSENCPKVI SCEFAHNSNW YITFQSDTDA QQAFKYLREE VKTFQGKPIM ARIKAINTFF AKNGYRLMD

Formula weight

20764.63 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 74.3 %, Completeness (bb): 78.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.3 % (1622 of 2183)	76.3 % (875 of 1147)	69.3 % (586 of 846)	84.7 % (161 of 190)
Backbone	78.6 % (832 of 1058)	88.1 % (312 of 354)	67.9 % (362 of 533)	92.4 % (158 of 171)
Sidechain	73.2 % (951 of 1300)	70.5 % (559 of 793)	79.7 % (389 of 488)	15.8 % (3 of 19)
Aromatic	48.2 % (79 of 164)	48.8 % (40 of 82)	46.3 % (37 of 80)	100.0 % (2 of 2)
Methyl	93.1 % (175 of 188)	92.6 % (87 of 94)	93.6 % (88 of 94)

1. LARP4A

QSNSAVSTED LKECLKKQLE FCFSRENLSK DLYLISQMDS DQFIPIWTVA NMEEIKKLTT DPDLILEVLR SSPMVQVDEK GEKVRPSHKR CIVILREIPE TTPIEEVKGL FKSENCPKVI SCEFAHNSNW YITFQSDTDA QQAFKYLREE VKTFQGKPIM ARIKAINTFF AKNGYRLMD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.25

#	Name	Isotope labeling	Concentration
1	LARP4A	[U-100% 13C; U-100% 15N]	400 uM
2	LARP4A	[U-100% 15N]	400 uM
3	TRIS	natural abundance	20 mM
4	KCL	natural abundance	100 mM
5	DTT	natural abundance	1 mM
6	EDTA	natural abundance	0.2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6I9B, Strand ID: A Detail

Release date

2018-10-28

Citation

Resonance assignment of human LARP4A La module
Cruz-Gallardo, I., Martino, L., Trotta, R., De Tito, S., Atkinson, A.R., Conte, M.R.
Biomol. NMR Assign. (2019), 13, 169-172, PubMed 30632004 , DOI 10.1007/s12104-019-09871-4 , Abstract

Related entities 1. LARP4A, : 1 : 2 : 15 entities Detail

Interaction partners 1. LARP4A, : 24 interactors Detail

More details for 24 interactors identified by 11 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC