BMRB: 27730

Structure determination of transmembrane- C-terminal fragment of UL49.5 protein from bovine herpesvirus 1 by NMR spectroscopy and molecular dynamics

Authors

Karska, N., Graul, M., Sikorska, E., Zhukov, I., Slusarz, M., Kasprzykowski, F., Lipinska, A., Rodziewicz-Motowidlo, S.

Assembly

TMC.BHV

Entity

1. TMC.BHV (polymer, Thiol state: not present), 40 monomers, 4212.935 Da Detail

VVFYVALTAV XVAVALYAYG LXFRLXGASG PNKKESRGRG

Formula weight

4212.935 Da

Source organism

Bovine alphaherpesvirus 1

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 90.5 %, Completeness (bb): 94.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	90.5 % (191 of 211)	90.5 % (191 of 211)
Backbone	94.9 % (74 of 78)	94.9 % (74 of 78)
Sidechain	88.0 % (117 of 133)	88.0 % (117 of 133)
Aromatic	90.9 % (20 of 22)	90.9 % (20 of 22)
Methyl	77.8 % (21 of 27)	77.8 % (21 of 27)

1. TMC.BHV

VVFYVALTAV XVAVALYAYG LXFRLXGASG PNKKESRGRG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5, Details NMR experiments were performed using 100 mM DPC-d38 in water (90%:10% H2O:D2O).

#	Name	Isotope labeling	Type	Concentration
1	TMC.BHV	natural abundance		2.7 mM
2	DPC	natural abundance		100 mM
3	Acetic Acid-d4	natural abundance		0.01 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 6QBJ, Strand ID: A Detail

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Release date

2018-12-16

Citation

Structure determination of UL49.5 transmembrane protein from bovine herpesvirus 1 by NMR spectroscopy and molecular dynamics
Karska, N., Graul, M., Sikorska, E., Zhukov, I., Slusarz, M.J., Kasprzykowski, F., Lipinska, A.D., Rodziewicz-Motowidlo, S.
Biochim. Biophys. Acta. Biomembr. (2019), 1861, 926-938, PubMed 30772281 , DOI 10.1016/j.bbamem.2019.02.005 , Abstract

Related entities 1. TMC.BHV, : 1 : 1 entities Detail

Interaction partners 1. TMC.BHV, : 6 interactors Detail

Experiments performed 4 experiments Detail