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Solution Structure of the RAZUL domain from 26S proteasome subunit hRpn10/S5a complexed with the AZUL domain from E3 ligase E6AP/UBE3A
Authors
Chen, X., Walters, K.J.
Assembly
proteasome proteins
Entity
1. proteasome proteins, entity 1 (polymer, Thiol state: all free), 73 monomers, 7959.507 Da Detail

SADIDASSAM DTSEPAKEED DYDVMQDPEF LQSVLENLPG VDPNNEAIRN AMGSLASQAT KDGKKDKKEE DKK


2. proteasome proteins, entity 2 (polymer, Thiol state: all free), 64 monomers, 7200.241 Da Detail

MKRAAAKHLI ERYYHQLTEG CGNEACTNEF CASCPTFLRM DNNAAAIKAL ELYKINAKLC DPHP


Total weight
15159.748 Da
Max. entity weight
7959.507 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 96.5 %, Completeness (bb): 99.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All96.5 % (1480 of 1534)98.0 % (788 of 804)93.5 % (549 of 587)100.0 % (143 of 143)
Backbone99.4 % (803 of 808)98.5 % (268 of 272)99.8 % (405 of 406)100.0 % (130 of 130)
Sidechain94.3 % (809 of 858)97.7 % (520 of 532)88.2 % (276 of 313)100.0 % (13 of 13)
Aromatic44.6 % (33 of 74)89.2 % (33 of 37) 0.0 % (0 of 37)
Methyl98.3 % (116 of 118)96.6 % (57 of 59)100.0 % (59 of 59)

1. entity 1

SADIDASSAM DTSEPAKEED DYDVMQDPEF LQSVLENLPG VDPNNEAIRN AMGSLASQAT KDGKKDKKEE DKK

2. entity 2

MKRAAAKHLI ERYYHQLTEG CGNEACTNEF CASCPTFLRM DNNAAAIKAL ELYKINAKLC DPHP

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N, 13C labeled hRpn10 mixed with unlabeled UBE3A


#NameIsotope labelingTypeConcentration
1entity_1[U-100% 13C; U-100% 15N]0.5 mM
2entity_2natural abundance0.75 mM
3MOPSnatural abundance10 mM
4sodium chloridenatural abundance50 mM
5DTTnatural abundance10 mM
6ZnSO4natural abundance10 uM
7sodium azidenatural abundance0.1 %
8pefablocnatural abundance1 mM
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N, 13C labeled hRpn10 mixed with unlabeled UBE3A


#NameIsotope labelingTypeConcentration
9entity_1[U-100% 13C; U-100% 15N]0.5 mM
10entity_2natural abundance0.75 mM
11MOPSnatural abundance10 mM
12sodium chloridenatural abundance50 mM
13DTTnatural abundance10 mM
14ZnSO4natural abundance10 uM
15sodium azidenatural abundance0.1 %
16pefablocnatural abundance1 mM
Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N, 13C labeled UBE3A mixed with unlabeled hRpn10


#NameIsotope labelingTypeConcentration
17entity_2[U-100% 13C; U-100% 15N]0.5 mM
18entity_1natural abundance0.75 mM
19MOPSnatural abundance10 mM
20sodium chloridenatural abundance50 mM
21DTTnatural abundance10 mM
22ZnSO4natural abundance10 uM
23sodium azidenatural abundance0.1 %
24pefablocnatural abundance1 mM
Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N labeled UBE3A mixed with unlabeled hRpn10


#NameIsotope labelingTypeConcentration
25entity_2[U-100% 15N]0.5 mM
26entity_1natural abundance0.75 mM
27MOPSnatural abundance10 mM
28sodium chloridenatural abundance50 mM
29DTTnatural abundance10 mM
30ZnSO4natural abundance10 uM
31sodium azidenatural abundance0.1 %
32pefablocnatural abundance1 mM
Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N, 13C labeled UBE3A mixed with unlabeled hRpn10


#NameIsotope labelingTypeConcentration
33entity_2[U-100% 15N]0.5 mM
34entity_1natural abundance0.75 mM
35MOPSnatural abundance10 mM
36sodium chloridenatural abundance50 mM
37DTTnatural abundance10 mM
38ZnSO4natural abundance10 uM
39sodium azidenatural abundance0.1 %
40pefablocnatural abundance1 mM

Protein Blocks Logo
Calculated from 15 models in PDB: 6U19, Strand ID: A, B Detail


Release date
2019-04-08
Citation
Structure of E3 ligase E6AP with a proteasome-binding site provided by substrate receptor hRpn10
Buel, G.R., Chen, X., Chari, R., O'Neill, M.J., Ebelle, D.L., Jenkins, C., Sridharan, V., Tarasov, S.G., Tarasova, N.I., Andresson, T., Walters, K.J.
Nat. Commun. (2020), 11, 1291-1291, PubMed 32157086 , DOI 10.1038/s41467-020-15073-7 ,
Related entities 1. proteasome proteins, entity 1, : 1 : 19 : 14 entities Detail
Related entities 2. proteasome proteins, entity 2, : 7 : 1 : 6 entities Detail
Interaction partners 1. proteasome proteins, entity 1, : 87 : 1 interactors Detail
Interaction partners 2. proteasome proteins, entity 2, : 3 interactors Detail
Experiments performed 22 experiments Detail
Chemical shift validation 4 contents Detail