Solution Structure of the RAZUL domain from 26S proteasome subunit hRpn10/S5a complexed with the AZUL domain from E3 ligase E6AP/UBE3A
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.5 % (1480 of 1534) | 98.0 % (788 of 804) | 93.5 % (549 of 587) | 100.0 % (143 of 143) |
Backbone | 99.4 % (803 of 808) | 98.5 % (268 of 272) | 99.8 % (405 of 406) | 100.0 % (130 of 130) |
Sidechain | 94.3 % (809 of 858) | 97.7 % (520 of 532) | 88.2 % (276 of 313) | 100.0 % (13 of 13) |
Aromatic | 44.6 % (33 of 74) | 89.2 % (33 of 37) | 0.0 % (0 of 37) | |
Methyl | 98.3 % (116 of 118) | 96.6 % (57 of 59) | 100.0 % (59 of 59) |
1. entity 1
SADIDASSAM DTSEPAKEED DYDVMQDPEF LQSVLENLPG VDPNNEAIRN AMGSLASQAT KDGKKDKKEE DKK2. entity 2
MKRAAAKHLI ERYYHQLTEG CGNEACTNEF CASCPTFLRM DNNAAAIKAL ELYKINAKLC DPHPSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N, 13C labeled hRpn10 mixed with unlabeled UBE3A
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
2 | entity_2 | natural abundance | 0.75 mM | |
3 | MOPS | natural abundance | 10 mM | |
4 | sodium chloride | natural abundance | 50 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | ZnSO4 | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.1 % | |
8 | pefabloc | natural abundance | 1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N, 13C labeled hRpn10 mixed with unlabeled UBE3A
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | entity_1 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
10 | entity_2 | natural abundance | 0.75 mM | |
11 | MOPS | natural abundance | 10 mM | |
12 | sodium chloride | natural abundance | 50 mM | |
13 | DTT | natural abundance | 10 mM | |
14 | ZnSO4 | natural abundance | 10 uM | |
15 | sodium azide | natural abundance | 0.1 % | |
16 | pefabloc | natural abundance | 1 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N, 13C labeled UBE3A mixed with unlabeled hRpn10
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | entity_2 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
18 | entity_1 | natural abundance | 0.75 mM | |
19 | MOPS | natural abundance | 10 mM | |
20 | sodium chloride | natural abundance | 50 mM | |
21 | DTT | natural abundance | 10 mM | |
22 | ZnSO4 | natural abundance | 10 uM | |
23 | sodium azide | natural abundance | 0.1 % | |
24 | pefabloc | natural abundance | 1 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N labeled UBE3A mixed with unlabeled hRpn10
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | entity_2 | [U-100% 15N] | 0.5 mM | |
26 | entity_1 | natural abundance | 0.75 mM | |
27 | MOPS | natural abundance | 10 mM | |
28 | sodium chloride | natural abundance | 50 mM | |
29 | DTT | natural abundance | 10 mM | |
30 | ZnSO4 | natural abundance | 10 uM | |
31 | sodium azide | natural abundance | 0.1 % | |
32 | pefabloc | natural abundance | 1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N, 13C labeled UBE3A mixed with unlabeled hRpn10
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
33 | entity_2 | [U-100% 15N] | 0.5 mM | |
34 | entity_1 | natural abundance | 0.75 mM | |
35 | MOPS | natural abundance | 10 mM | |
36 | sodium chloride | natural abundance | 50 mM | |
37 | DTT | natural abundance | 10 mM | |
38 | ZnSO4 | natural abundance | 10 uM | |
39 | sodium azide | natural abundance | 0.1 % | |
40 | pefabloc | natural abundance | 1 mM |