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BMRB: 27880

1H, 13C, 15N Backbone chemical Shift Assignment of the RYMV-encoded viral supressor of RNA silencing P1 protein

Authors

Yang, Y., Gillet, F., Hoh, F., Brugidou, C., Vignols, F.

Assembly

RYMV P1

Entity

1. RYMV P1 (polymer, Thiol state: free and other bound), 157 monomers, 17928.80 Da Detail

MTRLEVLIRP TEQTAAKANA VGYTHALTWV WHSQTWDVDS VRDPSLRADF NPEKVGWVSV SFACTQCTAH YYTSEQVKYF TNIPPVHFDV VCADCERSVQ LDDEIDREHQ ERNAEISACN ARALSEGRPA SLVYLSRDAC DIPEHSGRCR FVKYLNF

2. RYMV P1, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

18059.62 Da

Max. entity weight

17928.8 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS64:SG	2:ZN1:ZN
2	na	sing	1:CYS67:SG	2:ZN1:ZN
3	na	sing	1:CYS92:SG	2:ZN1:ZN
4	na	sing	1:CYS95:SG	2:ZN1:ZN
5	na	sing	1:HIS109:HE1	2:ZN1:ZN
6	na	sing	1:CYS140:SG	2:ZN1:ZN
7	na	sing	1:HIS145:HE1	2:ZN1:ZN
8	na	sing	1:CYS149:SG	2:ZN1:ZN

Source organism

Sobemovirus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 53.5 %, Completeness (bb): 93.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	53.5 % (959 of 1792)	39.0 % (360 of 922)	63.5 % (447 of 704)	91.6 % (152 of 166)
Backbone	93.2 % (865 of 928)	94.9 % (295 of 311)	91.9 % (429 of 467)	94.0 % (141 of 150)
Sidechain	23.1 % (235 of 1017)	10.6 % (65 of 611)	40.8 % (159 of 390)	68.8 % (11 of 16)
Aromatic	4.4 % (8 of 180)	4.4 % (4 of 90)	4.7 % (4 of 86)	0.0 % (0 of 4)
Methyl	21.1 % (35 of 166)	13.3 % (11 of 83)	28.9 % (24 of 83)

1. RYMV P1

MTRLEVLIRP TEQTAAKANA VGYTHALTWV WHSQTWDVDS VRDPSLRADF NPEKVGWVSV SFACTQCTAH YYTSEQVKYF TNIPPVHFDV VCADCERSVQ LDDEIDREHQ ERNAEISACN ARALSEGRPA SLVYLSRDAC DIPEHSGRCR FVKYLNF

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 300 (±0.5) K, pH 8 (±0.1)

#	Name	Isotope labeling	Concentration
1	RYMV P1	[U-99% 13C; U-99% 15N]	350 (±50.0) uM
2	ZnSO4	natural abundance	350 uM
3	DSS	natural abundance	50 uM
4	sodium chloride	natural abundance	100 mM
5	Na2SO4	natural abundance	60 mM
6	TRIS	natural abundance	15 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Release date

2019-04-16

Citation

NMR chemical shift backbone assignment of the viral protein P1 encoded by the African Rice Yellow Mottle Virus
Yang, Y., Poignavent, V., Gillet, F., Hoh, F., Brugidou, C., Vignols, F., Demene, H.
Biomol. NMR Assign. (2019), 13, 345-348, PubMed 31346897 , DOI 10.1007/s12104-019-09903-z , Abstract

Related entities 1. RYMV P1, : 9 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC