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BMRB: 27989

6YTS

Backbone and side-chain chemical shift assignments of the ribosome-inactivating protein trichobakin (TBK) in solution

Authors

Britikov, V.V., Britikova, E.V., Bocharov, E.V., Urban, A.S., Lesovoy, D.M., Le, T., Phan, V., Usanov, S.A., Arseniev, A.S.

Assembly

trichobakin

Entity

1. trichobakin (polymer, Thiol state: not present), 247 monomers, 27198.59 Da Detail

DVSFRLSGAT SSSYGVFISN LRKALPYERK LYDIPLLRST LPGSQRYALI HLTNYADETI SVAIDVTNVY VMGYRAGDTS YFFNEASATE AAKYVFKDAK RKVTLPYSGN YERLQIAAGK IRENIPLGLP ALDSAITTLF YYNANSAASA LMVLIQSTSE AARYKFIEQQ IGKRVDKTFL PSLAIISLEN SWSALSKQIQ IASTNNGQFE TPVVLINAQN QRVTITNVDA GVVTSNIALL PNRNNMA

Formula weight

27198.59 Da

Source organism

Trichosanthes sp. Bac Kan 8-98

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.6 %, Completeness: 89.7 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.7 % (2558 of 2852)	89.3 % (1309 of 1466)	89.2 % (998 of 1119)	94.0 % (251 of 267)
Backbone	97.0 % (1420 of 1464)	96.4 % (478 of 496)	97.5 % (712 of 730)	96.6 % (230 of 238)
Sidechain	84.2 % (1368 of 1624)	85.7 % (831 of 970)	82.6 % (516 of 625)	72.4 % (21 of 29)
Aromatic	55.5 % (121 of 218)	75.2 % (82 of 109)	35.2 % (38 of 108)	100.0 % (1 of 1)
Methyl	89.9 % (293 of 326)	90.2 % (147 of 163)	89.6 % (146 of 163)

1. trichobakin

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	trichobakin	[U-100% 13C; U-100% 15N]		0.8 mM
2	potassium phosphate buffer	natural abundance		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

Release date

2019-07-29

Citation

Backbone and side-chain chemical shift assignments for the ribosome-inactivating protein trichobakin (TBK)
Britikov, V.V., Britikova, E.V., Urban, A.S., Lesovoy, D.M., Le, T., Van Phan, C., Usanov, S.A., Arseniev, A.S., Bocharov, E.V.
Biomol. NMR Assign. (2019), 14, 55-61, PubMed 31734904 , DOI 10.1007/s12104-019-09920-y , Abstract

Related entities 1. trichobakin, : 1 : 1 : 1 : 146 entities Detail

Interaction partners 1. trichobakin, : 3 interactors Detail

More details for 3 interactors identified by 1 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC