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BMRB: 28014

7NAS

Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments of RimP from E. coli

Authors

Schedlbauer, A., Kaminishi, T., Iturrioz, I., Ochoa Lizarralde, B., Gil-Carton, D., de Astigarraga, E., Diercks, T., Fucini, P., Connell, S.

Assembly

RimP

Entity

1. RimP (polymer, Thiol state: all free), 151 monomers, 16721.84 Da Detail

MSTLEQKLTE MITAPVEALG FELVGIEFIR GRTSTLRIYI DSEDGINVDD CADVSHQVSA VLDVEDPITV AYNLEVSSPG LDRPLFTAEH YARFVGEEVT LVLRMAVQNR RKWQGVIKAV DGEMITVTVE GKDEVFALSN IQKANLVPHF A

Formula weight

16721.84 Da

Source organism

Escherichia coli

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 89.3 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.3 % (1542 of 1727)	89.2 % (785 of 880)	89.7 % (612 of 682)	87.9 % (145 of 165)
Backbone	96.8 % (867 of 896)	96.4 % (295 of 306)	97.7 % (434 of 444)	94.5 % (138 of 146)
Sidechain	83.7 % (814 of 973)	85.4 % (490 of 574)	83.4 % (317 of 380)	36.8 % (7 of 19)
Aromatic	11.1 % (12 of 108)	22.2 % (12 of 54)	0.0 % (0 of 53)	0.0 % (0 of 1)
Methyl	93.9 % (199 of 212)	93.4 % (99 of 106)	94.3 % (100 of 106)

1. RimP

MSTLEQKLTE MITAPVEALG FELVGIEFIR GRTSTLRIYI DSEDGINVDD CADVSHQVSA VLDVEDPITV AYNLEVSSPG LDRPLFTAEH YARFVGEEVT LVLRMAVQNR RKWQGVIKAV DGEMITVTVE GKDEVFALSN IQKANLVPHF A

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

Release date

2019-09-10

Citation

Backbone and sidechain NMR assignments for the ribosome maturation factor RimP from Escherichia coli
Schedlbauer, A., Ochoa-Lizarralde, B., Iturrioz, I., Capuni, R., Diercks, T., de Astigarraga, E., Fucini, P., Connell, S.R.
Biomol. NMR Assign. (2020), 14, 189-193, PubMed 32303998 , DOI 10.1007/s12104-020-09943-w , Abstract

Related entities 1. RimP, : 1 : 7 : 2 : 105 entities Detail

Experiments performed 13 experiments Detail

1 2D 15N TROSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

2 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

3 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

4 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

5 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

6 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

7 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

9 3D CNH NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

10 3D HNH NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

11 3D HCH NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

12 3D HNCAHA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

13 3D HN(CO)CAHA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Type	Concentration
1	RimP	[U-98% 13C; U-98% 15N]		600 uM
2	HEPES	natural abundance		10 mM
3	MgCl2	natural abundance		6 mM
4	NH4Cl	natural abundance		30 mM
5	TCEP	natural abundance		75 uM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr28014_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr28014_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1755		98.0 (chain: 1, length: 151)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 151, Sequence coverage: 98.0%
- Total number of assignments
  - ¹H chemical shifts: 991
  - ¹³C chemical shifts: 619
  - ¹⁵N chemical shifts: 145
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1