1H, 13C, 15N backbone and side-chain assignment of the native form of UbcH7 (UBE2L3)
MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.9 % (1803 of 1900) | 95.1 % (961 of 1010) | 93.6 % (686 of 733) | 99.4 % (156 of 157) |
Backbone | 98.4 % (882 of 896) | 98.0 % (293 of 299) | 98.5 % (450 of 457) | 99.3 % (139 of 140) |
Sidechain | 92.8 % (1070 of 1153) | 94.0 % (668 of 711) | 90.6 % (385 of 425) | 100.0 % (17 of 17) |
Aromatic | 55.6 % (79 of 142) | 63.4 % (45 of 71) | 46.4 % (32 of 69) | 100.0 % (2 of 2) |
Methyl | 99.3 % (145 of 146) | 98.6 % (72 of 73) | 100.0 % (73 of 73) |
1. UbcH7
MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | UbcH7 | [U-99% 15N] | 1.0 mM | |
2 | K2HPO4/KH2PO4 | natural abundance | 50.0 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | UbcH7 | [U-98% 13C; U-98% 15N] | 1.0 mM | |
4 | K2HPO4/KH2PO4 | natural abundance | 50.0 mM |