BMRBj - BMREntryMaster

Found 1 Document (0.264 seconds)

BMRB: 28094

Solution NMR structure of 5'UTR of Stem loop B in DENV4

Authors

Sharma, S., Varani, G.

Assembly

DENV4

Entity

1. DENV4 (polymer, Thiol state: not present), 41 monomers, 13317.89 Da Detail

GGUUUGUUUG AAUAGAGAGC AGAUCUCUGG AAAAAUGAAC C

Formula weight

13317.89 Da

Source organism

Flavivirus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.3 %, Completeness (bb): 94.7 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	94.3 % (610 of 647)	93.7 % (327 of 349)	96.0 % (265 of 276)	81.8 % (18 of 22)
Suger, PO4	94.7 % (427 of 451)	94.3 % (232 of 246)	95.1 % (195 of 205)
Nucleobase	93.4 % (183 of 196)	92.2 % (95 of 103)	98.6 % (70 of 71)	81.8 % (18 of 22)
Aromatic	95.2 % (177 of 186)	95.7 % (89 of 93)	98.6 % (70 of 71)	81.8 % (18 of 22)

1. DENV4

GGUUUGUUUG AAUAGAGAGC AGAUCUCUGG AAAAAUGAAC C

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±5) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	DENV4	[U-99% 13C; U-99% 15N]		0.75 mM
2	sodium phosphate	natural abundance		20 mM

Release date

2020-03-08

Citation

NMR structure of Dengue West Nile viruses stem-loop B: A key cis-acting element for flavivirus replication
Sharma, S., Varani, G.
Biochem. Biophys. Res. Commun. (2020), 531, 522-527, PubMed 32807496 , DOI 10.1016/j.bbrc.2020.07.115 , Abstract

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr28094_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	610		100.0 (chain: 1, length: 41)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	39	100.0
¹³C chemical shifts	39	38	97.4

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Found 1 Document (0.264 seconds)

BMRB: 28094

Sample

Experiments performed 5 experiments Detail

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Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	349	327	93.7
¹³C chemical shifts	276	265	96.0
¹⁵N chemical shifts	22	18	81.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	246	232	94.3
¹³C chemical shifts	205	195	95.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	103	95	92.2
¹³C chemical shifts	71	70	98.6
¹⁵N chemical shifts	22	18	81.8